Measurement of homogeneous kinase activity for cell lysates based on the aggregation of gold nanoparticles

Jun Oishi; Yoji Asami; Takeshi Mori; Jeong Hun Kang; Miharu Tanabe; Takuro Niidome; Yoshiki Katayama

doi:10.1002/cbic.200700086

Measurement of homogeneous kinase activity for cell lysates based on the aggregation of gold nanoparticles

Jun Oishi, Yoji Asami, Takeshi Mori, Jeong Hun Kang, Miharu Tanabe, Takuro Niidome, Yoshiki Katayama

Research output: Contribution to journal › Article › peer-review

74 Citations (Scopus)

Abstract

When the charge sticks. A kinase activity screening system based on the aggregation of gold nanoparticles induced by cationic substrate peptides as coagulants has been constructed. The system can conduct and monitor kinase reactions under homogeneous conditions and has been successfully applied to the kinase assay of cell lysates. (Figure Presented).

Original language	English
Pages (from-to)	875-879
Number of pages	5
Journal	ChemBioChem
Volume	8
Issue number	8
DOIs	https://doi.org/10.1002/cbic.200700086
Publication status	Published - May 25 2007

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Medicine
Molecular Biology
Organic Chemistry

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10.1002/cbic.200700086

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title = "Measurement of homogeneous kinase activity for cell lysates based on the aggregation of gold nanoparticles",

abstract = "When the charge sticks. A kinase activity screening system based on the aggregation of gold nanoparticles induced by cationic substrate peptides as coagulants has been constructed. The system can conduct and monitor kinase reactions under homogeneous conditions and has been successfully applied to the kinase assay of cell lysates. (Figure Presented).",

author = "Jun Oishi and Yoji Asami and Takeshi Mori and Kang, {Jeong Hun} and Miharu Tanabe and Takuro Niidome and Yoshiki Katayama",

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AU - Oishi, Jun

AU - Asami, Yoji

AU - Mori, Takeshi

AU - Kang, Jeong Hun

AU - Tanabe, Miharu

AU - Niidome, Takuro

AU - Katayama, Yoshiki

PY - 2007/5/25

Y1 - 2007/5/25

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AB - When the charge sticks. A kinase activity screening system based on the aggregation of gold nanoparticles induced by cationic substrate peptides as coagulants has been constructed. The system can conduct and monitor kinase reactions under homogeneous conditions and has been successfully applied to the kinase assay of cell lysates. (Figure Presented).

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Measurement of homogeneous kinase activity for cell lysates based on the aggregation of gold nanoparticles

Abstract

All Science Journal Classification (ASJC) codes

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