Measurement and correlation of partition coefficients of hydrolytic enzymes for dextran+poly (ethylene glycol) + water aqueous two-phase systems at 20°C

T. Furuya; Y. Iwai; Y. Tanaka; H. Uchida; S. Yamada; Y. Arai

doi:10.1016/0378-3812(95)02746-2

Measurement and correlation of partition coefficients of hydrolytic enzymes for dextran+poly (ethylene glycol) + water aqueous two-phase systems at 20°C

T. Furuya, Y. Iwai, Y. Tanaka, H. Uchida, S. Yamada, Y. Arai

Molecular and Biochemical Systems Engineering

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

The partition coefficients of hydrolytic enzymes, α-amylase, β-amylase and glucoamylase for the dextran (DEX)+poly(ethylene glycol) (PEG)+water aqueous two-phase systems with various polymer molecular weights were measured at 20°C. The partition coefficients obtained were correlated by using a mod fied Flory-Huggins equation which was empirically proposed for aqueous systems. The interaction parameters required were determined by fitting the model to the experimental data. The partition coefficients could be correlated with good agreement.

Original language	English
Pages (from-to)	115-128
Number of pages	14
Journal	Fluid Phase Equilibria
Volume	110
Issue number	1-2
DOIs	https://doi.org/10.1016/0378-3812(95)02746-2
Publication status	Published - Sept 1995

All Science Journal Classification (ASJC) codes

Chemical Engineering(all)
Physics and Astronomy(all)
Physical and Theoretical Chemistry

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10.1016/0378-3812(95)02746-2

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title = "Measurement and correlation of partition coefficients of hydrolytic enzymes for dextran+poly (ethylene glycol) + water aqueous two-phase systems at 20°C",

abstract = "The partition coefficients of hydrolytic enzymes, α-amylase, β-amylase and glucoamylase for the dextran (DEX)+poly(ethylene glycol) (PEG)+water aqueous two-phase systems with various polymer molecular weights were measured at 20°C. The partition coefficients obtained were correlated by using a mod fied Flory-Huggins equation which was empirically proposed for aqueous systems. The interaction parameters required were determined by fitting the model to the experimental data. The partition coefficients could be correlated with good agreement.",

author = "T. Furuya and Y. Iwai and Y. Tanaka and H. Uchida and S. Yamada and Y. Arai",

note = "Funding Information: We gratefully acknowledge the financial support provided by the Grant-in-Aid for Encouragement of Young Scientists (1993, 05750676), The Ministry of Education, Science and Culture, Japan. We also thank Mr. K. Nakada, and Ms. J. Zhu for their support in this experiment.",

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T1 - Measurement and correlation of partition coefficients of hydrolytic enzymes for dextran+poly (ethylene glycol) + water aqueous two-phase systems at 20°C

AU - Furuya, T.

AU - Iwai, Y.

AU - Tanaka, Y.

AU - Uchida, H.

AU - Yamada, S.

AU - Arai, Y.

N1 - Funding Information: We gratefully acknowledge the financial support provided by the Grant-in-Aid for Encouragement of Young Scientists (1993, 05750676), The Ministry of Education, Science and Culture, Japan. We also thank Mr. K. Nakada, and Ms. J. Zhu for their support in this experiment.

PY - 1995/9

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N2 - The partition coefficients of hydrolytic enzymes, α-amylase, β-amylase and glucoamylase for the dextran (DEX)+poly(ethylene glycol) (PEG)+water aqueous two-phase systems with various polymer molecular weights were measured at 20°C. The partition coefficients obtained were correlated by using a mod fied Flory-Huggins equation which was empirically proposed for aqueous systems. The interaction parameters required were determined by fitting the model to the experimental data. The partition coefficients could be correlated with good agreement.

AB - The partition coefficients of hydrolytic enzymes, α-amylase, β-amylase and glucoamylase for the dextran (DEX)+poly(ethylene glycol) (PEG)+water aqueous two-phase systems with various polymer molecular weights were measured at 20°C. The partition coefficients obtained were correlated by using a mod fied Flory-Huggins equation which was empirically proposed for aqueous systems. The interaction parameters required were determined by fitting the model to the experimental data. The partition coefficients could be correlated with good agreement.

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Measurement and correlation of partition coefficients of hydrolytic enzymes for dextran+poly (ethylene glycol) + water aqueous two-phase systems at 20°C

Abstract

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