TY - JOUR
T1 - Maturation-promoting factor and p34cdc2 kinase during oocyte maturation of the Japanese quail
AU - Mori, M.
AU - Yamashita, M.
AU - Yoshikuni, M.
AU - Fukada, S.
AU - Nagahama, Y.
N1 - Funding Information:
We are grateful to Dr. P. Nurse for critical reading of the manuscript and to Dr. J. Hayles who prepared the bacterial strain overex-pressing ~13~‘. We also thank Dr. I. G. Gleadall for helpful comments on the manuscript. This research was in part supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan (02102010 to Y.N.), the Naito Foundation, and the Japan Health Sciences Foundation.
PY - 1991/7
Y1 - 1991/7
N2 - Maturation-promoting factor and a homolog of fission yeast cdc2+ gene product (p34cdc2) were investigated during the final 24 hr of maturation of quail oocytes. Kinase activity of p34cdc2 in the oocyte germinal disk (GD) increased 15 times at maturation. Two bands, at 32 and 34 kDa, were detected in immature oocytes by immunoblotting of SDS-PAGE with anti-p34cdc2 monoclonal antibody. A new band, which is close to the 32-kDa band but with a slightly faster mobility, appeared during maturation. No p34cdc2 could be detected outside the GD. Microinjection of GD extract from mature oocytes caused maturation of Xenopus oocytes.
AB - Maturation-promoting factor and a homolog of fission yeast cdc2+ gene product (p34cdc2) were investigated during the final 24 hr of maturation of quail oocytes. Kinase activity of p34cdc2 in the oocyte germinal disk (GD) increased 15 times at maturation. Two bands, at 32 and 34 kDa, were detected in immature oocytes by immunoblotting of SDS-PAGE with anti-p34cdc2 monoclonal antibody. A new band, which is close to the 32-kDa band but with a slightly faster mobility, appeared during maturation. No p34cdc2 could be detected outside the GD. Microinjection of GD extract from mature oocytes caused maturation of Xenopus oocytes.
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U2 - 10.1016/0012-1606(91)90465-F
DO - 10.1016/0012-1606(91)90465-F
M3 - Article
C2 - 2060707
AN - SCOPUS:0025892841
SN - 0012-1606
VL - 146
SP - 246
EP - 249
JO - Developmental Biology
JF - Developmental Biology
IS - 1
ER -