TY - JOUR
T1 - Matrix-assisted laser desorption/ionization mass spectrometry-guided visualization analysis of intestinal absorption of acylated anthocyanins in Sprague-Dawley rats
AU - Hahm, Tae Hun
AU - Tanaka, Mitsuru
AU - Nguyen, Huu Nghi
AU - Tsutsumi, Ayaka
AU - Aizawa, Koichi
AU - Matsui, Toshiro
N1 - Funding Information:
This study was supported in part by a grant-in-aid from the Ministry of Education, Science, Sports, and Culture of Japan (No. 18K05534 to M.T. and No. 18H02157 to T.M.), and in part by a grant-in-aid from the Tojuro lijima Foundation for Food Science and Technology to M.T. The authors thank the Center for Advanced Instrumental and Educational Supports, Faculty of Agriculture, Kyushu University for technical support on cryosectioning of intestinal tissues.
Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2021/1/1
Y1 - 2021/1/1
N2 - It is unknown whether intestinal absorption of acylated anthocyanins occurs in their intact or metabolized form. In this study, with the aid of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) imaging, intestinal absorption of acylated anthocyanins was visually investigated. Anthocyanin extracts from purple carrots were orally administered to Sprague-Dawley rats. Acylated cyanidins were absorbed into portal and circulating blood systems in their intact form, and aglycon; cyanidin 3-O-(6-O-feruloyl-β-D-glucopyranosyl)-(1 → 6)-[β-D-xylopyranosyl-(1 → 2)]-β-D-galactopyranoside (Cy3XFGG), and showed a high absorption of 39.3 ± 0.1 pmol/mL-plasma at 60 min after administration. MALDI-MS imaging analysis of the rat jejunum membranes showed that an organic anion transporting polypeptide (OATP) transporter was involved in Cy3XFGG transport, while deacylated anthocyanins were incorporated through both the glucose transporter 2 and OATP routes. In conclusion, acylated anthocyanin, Cy3XFGG, can be absorbed in its intact form through intestinal OATP.
AB - It is unknown whether intestinal absorption of acylated anthocyanins occurs in their intact or metabolized form. In this study, with the aid of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) imaging, intestinal absorption of acylated anthocyanins was visually investigated. Anthocyanin extracts from purple carrots were orally administered to Sprague-Dawley rats. Acylated cyanidins were absorbed into portal and circulating blood systems in their intact form, and aglycon; cyanidin 3-O-(6-O-feruloyl-β-D-glucopyranosyl)-(1 → 6)-[β-D-xylopyranosyl-(1 → 2)]-β-D-galactopyranoside (Cy3XFGG), and showed a high absorption of 39.3 ± 0.1 pmol/mL-plasma at 60 min after administration. MALDI-MS imaging analysis of the rat jejunum membranes showed that an organic anion transporting polypeptide (OATP) transporter was involved in Cy3XFGG transport, while deacylated anthocyanins were incorporated through both the glucose transporter 2 and OATP routes. In conclusion, acylated anthocyanin, Cy3XFGG, can be absorbed in its intact form through intestinal OATP.
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U2 - 10.1016/j.foodchem.2020.127586
DO - 10.1016/j.foodchem.2020.127586
M3 - Article
C2 - 32707364
AN - SCOPUS:85088113674
SN - 0308-8146
VL - 334
JO - Food Chemistry
JF - Food Chemistry
M1 - 127586
ER -