TY - JOUR
T1 - Mass-tag technology for monitoring of protein kinase activity using mass spectrometry
AU - Sonoda, Tatsuhiko
AU - Shigaki, Syuhei
AU - Nagashima, Takeyuki
AU - Okitsu, Osamu
AU - Kita, Yasuhiro
AU - Murata, Masaharu
AU - Katayama, Yoshiki
N1 - Funding Information:
This work was supported by a grant-in-aid from the New Energy and Industrial Technology Development Organization (NEDO) and also by Regional Science Promotion Program, JST Co.
PY - 2004/2/23
Y1 - 2004/2/23
N2 - Monitoring of intracellular protein kinase activity is very important for fields involving diagnosis and drug screening. However, current methods, such as radiometry using 32P, or ELISA, are laborious and time-consuming. We have developed high-throughput assay system of protein kinase activity using mass-tagged substrate peptide probes and mass spectrometry. This assay system can easily evaluate target kinase activity and will potentially be able to simultaneously profile many protein kinase activities.
AB - Monitoring of intracellular protein kinase activity is very important for fields involving diagnosis and drug screening. However, current methods, such as radiometry using 32P, or ELISA, are laborious and time-consuming. We have developed high-throughput assay system of protein kinase activity using mass-tagged substrate peptide probes and mass spectrometry. This assay system can easily evaluate target kinase activity and will potentially be able to simultaneously profile many protein kinase activities.
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U2 - 10.1016/j.bmcl.2003.12.022
DO - 10.1016/j.bmcl.2003.12.022
M3 - Article
C2 - 15012979
AN - SCOPUS:0842346038
SN - 0960-894X
VL - 14
SP - 847
EP - 850
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
IS - 4
ER -