Mapping of ubiquitination sites on target proteins

Shigetsugu Hatakeyama, Masaki Matsumoto, Keiichi I. Nakayama

Research output: Contribution to journalReview articlepeer-review

9 Citations (Scopus)


Although the identification of ubiquitin-conjugated lysine residues on target proteins is extremely significant, to date it is generally quite difficult to identify ubiquitinated sites by usual mutation analysis. More recently, the technology of mass spectrometry is answering these difficult questions. In this chapter, we introduce the method of purification of ubiquitinated target proteins using affinity chromatography with anti-polyubiquitin antibody and the identification of ubiquitinated lysine residues on target proteins using mass spectrometry. Using these techniques, we can obtain comprehensive information about ubiquitinated proteins in various cells and tissues.

Original languageEnglish
Article number19
Pages (from-to)277-286
Number of pages10
JournalMethods in enzymology
Publication statusPublished - 2005

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology


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