@article{fca3246c2c6c4e298de807d63c2ddded,
title = "Mammalian mitochondrial DNA replication intermediates are essentially duplex but contain extensive tracts of RNA/DNA hybrid",
abstract = "We demonstrate, using transmission electron microscopy and immunopurification with an antibody specific for RNA/DNA hybrid, that intact mitochondrial DNA replication intermediates are essentially duplex throughout their length but contain extensive RNA tracts on one strand. However, the extent of preservation of RNA in such molecules is highly dependent on the preparative method used. These findings strongly support the strand-coupled model of mitochondrial DNA replication involving RNA incorporation throughout the lagging strand.",
author = "Pohjoism{\"a}ki, {Jaakko L.O.} and Holmes, {J. Bradley} and Wood, {Stuart R.} and Yang, {Ming Yao} and Takehiro Yasukawa and Aurelio Reyes and Bailey, {Laura J.} and Cluett, {Tricia J.} and Steffi Goffart and Smaranda Willcox and Rigby, {Rachel E.} and Jackson, {Andrew P.} and Spelbrink, {Johannes N.} and Griffith, {Jack D.} and Crouch, {Robert J.} and Jacobs, {Howard T.} and Holt, {Ian J.}",
note = "Funding Information: We thank JiaXiao Shen for technical assistance and Dr. Susana M. Cerritelli for her critique of the manuscript. The study was funded by the Medical Research Council , Academy of Finland , Juselius Foundation , Tampere University Hospital Medical Research Fund , National Institutes of Health (grant GM31819 and the Intramural Research Program of the Eunice Kennedy Shriver National Institute of Child Health and Human Development ), and the European Union (EUMITOCOMBAT project) . ",
year = "2010",
month = apr,
doi = "10.1016/j.jmb.2010.02.029",
language = "English",
volume = "397",
pages = "1144--1155",
journal = "Journal of Molecular Biology",
issn = "0022-2836",
publisher = "Academic Press Inc.",
number = "5",
}