TY - JOUR
T1 - Maintenance of Cartilaginous Gene Expression of Serially Subcultured Chondrocytes on Poly(2-Methoxyethyl Acrylate) Analogous Polymers
AU - Hoshiba, Takashi
AU - Maruyama, Hiroka
AU - Sato, Kazuhiro
AU - Endo, Chiho
AU - Kawazoe, Naoki
AU - Chen, Guoping
AU - Tanaka, Masaru
N1 - Funding Information:
T.H. and H.M. contributed equally to this work. This work was supported by a Grant-in-Aid for Young Scientists (A) (26702016) funded by MEXT, Japan and the Matching Planner Program (MP27115658712) funded by the Japan Science and Technology Agency (JST). Additionally, T.H. and M.T. were partially supported by the Center of Innovation (COI) Program from JST.
Publisher Copyright:
© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2017/12
Y1 - 2017/12
N2 - Chondrocytes are important for cartilage tissue engineering. However, dedifferentiation during chondrocyte subculture prevents the application of cartilage tissue engineering. Therefore, prevention of this dedifferentiation is required. Here, the possibility of poly(2-methoxyethyl acrylate) (PMEA) and its analogous polymers, poly(tetrahydrofurfuryl acrylate) (PTHFA) and poly(2-(2-methoxyethoxy) ethyl acrylate-co-butyl acrylate) (PMe2A), for chondrocyte subculture without dedifferentiation is examined. Chondrocytes spread on PTHFA and polyethylene terephthalate (PET), whereas their spreading is delayed on PMEA and PMe2A. When primary chondrocytes are subcultured on these polymers, the expression levels of cartilaginous genes are higher on PMEA and PMe2A than on PET and PTHFA. Integrin contribution to the initial cell adhesion is lower on PMEA and PMe2A than on PTHFA and PET. This low level of integrin contribution to cell adhesion may cause a delay in cell spreading and the maintenance of cartilaginous gene expression. These results indicate that PMEA and PMe2A may be favorable substrates for chondrocyte subculture and cartilage tissue engineering.
AB - Chondrocytes are important for cartilage tissue engineering. However, dedifferentiation during chondrocyte subculture prevents the application of cartilage tissue engineering. Therefore, prevention of this dedifferentiation is required. Here, the possibility of poly(2-methoxyethyl acrylate) (PMEA) and its analogous polymers, poly(tetrahydrofurfuryl acrylate) (PTHFA) and poly(2-(2-methoxyethoxy) ethyl acrylate-co-butyl acrylate) (PMe2A), for chondrocyte subculture without dedifferentiation is examined. Chondrocytes spread on PTHFA and polyethylene terephthalate (PET), whereas their spreading is delayed on PMEA and PMe2A. When primary chondrocytes are subcultured on these polymers, the expression levels of cartilaginous genes are higher on PMEA and PMe2A than on PET and PTHFA. Integrin contribution to the initial cell adhesion is lower on PMEA and PMe2A than on PTHFA and PET. This low level of integrin contribution to cell adhesion may cause a delay in cell spreading and the maintenance of cartilaginous gene expression. These results indicate that PMEA and PMe2A may be favorable substrates for chondrocyte subculture and cartilage tissue engineering.
UR - http://www.scopus.com/inward/record.url?scp=85037328270&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85037328270&partnerID=8YFLogxK
U2 - 10.1002/mabi.201700297
DO - 10.1002/mabi.201700297
M3 - Article
C2 - 29134785
AN - SCOPUS:85037328270
SN - 1616-5187
VL - 17
JO - Macromolecular Bioscience
JF - Macromolecular Bioscience
IS - 12
M1 - 1700297
ER -