TY - GEN
T1 - Magnetically driven micro-movable electrode for cell coupling
AU - Yamanishi, Y.
AU - Kawahara, T.
AU - Iyanagi, T.
AU - Hagiwara, M.
AU - Arai, Fumihito
PY - 2010
Y1 - 2010
N2 - We developed a novel method of cell coupling on a chip by using magnetically driven microtool (MMT). It is made of nickel and is used as an electrode as well as a cell pusher. The novelty of this device is the electrode itself whose tip part is supported by a spring and is positioned by external magnetic field. We propose a new cell coupling chip which provides larger coupling force of cells by using a physical motion of MMT. The operation of the microelectrode showed that the oocyte is deformed and the donor cell is successfully coupled physically with the oocyte. The contact area between the cells was increased, and which is good for consequent fusion process. The FEM analysis on the electric field density also showed that a high concentration between the membrane of oocyte and donor cell. The proposed method is suited for cell fusion on a chip, and will contribute to high throughput cell manipulation.
AB - We developed a novel method of cell coupling on a chip by using magnetically driven microtool (MMT). It is made of nickel and is used as an electrode as well as a cell pusher. The novelty of this device is the electrode itself whose tip part is supported by a spring and is positioned by external magnetic field. We propose a new cell coupling chip which provides larger coupling force of cells by using a physical motion of MMT. The operation of the microelectrode showed that the oocyte is deformed and the donor cell is successfully coupled physically with the oocyte. The contact area between the cells was increased, and which is good for consequent fusion process. The FEM analysis on the electric field density also showed that a high concentration between the membrane of oocyte and donor cell. The proposed method is suited for cell fusion on a chip, and will contribute to high throughput cell manipulation.
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M3 - Conference contribution
AN - SCOPUS:80052109133
SN - 9781618390622
T3 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
SP - 905
EP - 907
BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
T2 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Y2 - 3 October 2010 through 7 October 2010
ER -