TY - GEN
T1 - Magnetic manipulation of a retroviral vector using magnetite cationic liposomes
AU - Ito, Akira
AU - Takahashi, Tetsuya
AU - Kameyama, Yujiro
AU - Kawabe, Yoshinori
AU - Kamihira, Masamichi
PY - 2008
Y1 - 2008
N2 - In the present study, we used magnetite nanoparticles and magnetic force to concentrate the retroviral vectors in order to enhance the transduction efficiency and to enable their magnetic manipulation. Magnetite nanoparticles modified with Cationic liposomes were added to a solution containing a retroviral vector. The magnetic particles which captured the viral vectors were collected by a magnetic force, and seeded into target cells. The viral titer increased up to 55-fold and 3 x 108 IU/mL. Additionally, the magnetically labeled retroviral vectors can be directed to the desired regions for infection by applying magnetic fields, and micro-patterns of gene-transduced cell regions could be created on a cellular monolayer using micro-patterned magnetic concentrators. These results suggest that this new technique provides a promising approach to capture and concentrate viral vectors, thus achieving high transduction efficiency and the ability to deliver genes to a specifically injured site by applying a magnetic field.
AB - In the present study, we used magnetite nanoparticles and magnetic force to concentrate the retroviral vectors in order to enhance the transduction efficiency and to enable their magnetic manipulation. Magnetite nanoparticles modified with Cationic liposomes were added to a solution containing a retroviral vector. The magnetic particles which captured the viral vectors were collected by a magnetic force, and seeded into target cells. The viral titer increased up to 55-fold and 3 x 108 IU/mL. Additionally, the magnetically labeled retroviral vectors can be directed to the desired regions for infection by applying magnetic fields, and micro-patterns of gene-transduced cell regions could be created on a cellular monolayer using micro-patterned magnetic concentrators. These results suggest that this new technique provides a promising approach to capture and concentrate viral vectors, thus achieving high transduction efficiency and the ability to deliver genes to a specifically injured site by applying a magnetic field.
UR - http://www.scopus.com/inward/record.url?scp=62449179820&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=62449179820&partnerID=8YFLogxK
U2 - 10.1109/MHS.2008.4752479
DO - 10.1109/MHS.2008.4752479
M3 - Conference contribution
AN - SCOPUS:62449179820
SN - 9781424429196
T3 - 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008
SP - 367
EP - 371
BT - 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics"
T2 - 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation"
Y2 - 6 November 2008 through 9 November 2008
ER -