TY - JOUR
T1 - Lysosphingomyelin-elicited Ca2+ mobilization from rat brain microsomes
AU - Furuya, Shigeki
AU - Kurono, Sadamu
AU - Hirabayashi, Yoshio
N1 - Funding Information:
Acknowledgement We would like to thank Dr. K. Ono (Pharma Research Laboratory, Hoechst Japan Corp.) for a generous gift of VJ-41 antibody. We are grateful to Professor M. Ui (Tokyo Metropolitan Institute of Medical Science) and Dr. Fujioka (Ui Laboratory, RIKEN) for lending us a Hitachi spectrofluorometer, and to Professor Y. Nagai (Mitsubishi Kagaku Institute of Life Science) for encouragement of our work. This study was supported partially by the Grants-in-Aid for Scientific Research (no. 07780710 to S.F.) from the Ministry of Education, Science and Culture of Japan.
PY - 1996/9
Y1 - 1996/9
N2 - We have examined the Ca2+ release activity of sphingolipid-derivatives from rat brain microsomes using a Fura-2 cytofluorometric assay. Sphingosylphosphorylcholine, lysosphingomyelin, elicited a rapid Ca2+ release from both cerebral and cerebellar microsomes. Other compounds including sphingosine and sphingosine-1-phosphate were incapable of causing the Ca2+ release. The pharmacological properties suggest that the sphingosylphosphorylcholine-elicited Ca2+ mobilization is not mediated by inositol 1,4,5-triphosphate receptors. Immunocytochemical study showed the occurrence of sphingomyelin, a putative precursor for sphingosylphosphorylcholine, in the somatodendritic membrane domains of cerebellar neurons. These observations imply that sphingosylphosphorylcholine is a potent Ca2+ releaser in brain neurons.
AB - We have examined the Ca2+ release activity of sphingolipid-derivatives from rat brain microsomes using a Fura-2 cytofluorometric assay. Sphingosylphosphorylcholine, lysosphingomyelin, elicited a rapid Ca2+ release from both cerebral and cerebellar microsomes. Other compounds including sphingosine and sphingosine-1-phosphate were incapable of causing the Ca2+ release. The pharmacological properties suggest that the sphingosylphosphorylcholine-elicited Ca2+ mobilization is not mediated by inositol 1,4,5-triphosphate receptors. Immunocytochemical study showed the occurrence of sphingomyelin, a putative precursor for sphingosylphosphorylcholine, in the somatodendritic membrane domains of cerebellar neurons. These observations imply that sphingosylphosphorylcholine is a potent Ca2+ releaser in brain neurons.
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U2 - 10.1016/0929-7855(96)00539-1
DO - 10.1016/0929-7855(96)00539-1
M3 - Article
C2 - 8906576
AN - SCOPUS:0030226775
SN - 0929-7855
VL - 14
SP - 303
EP - 311
JO - Journal of Lipid Mediators and Cell Signalling
JF - Journal of Lipid Mediators and Cell Signalling
IS - 1-3
ER -