TY - JOUR
T1 - Lysosomal acid phosphatase is transported via endosomes to lysosomes
AU - Tanaka, Yoshitaka
AU - Yano, Shinji
AU - Okada, Kazutaka
AU - Ishikawa, Toyoko
AU - Himeno, Masaru
AU - Kato, Keitaro
N1 - Funding Information:
Acknowledgments: We wish to thank M. Ohara for helpful This study was supported in part by a Grant-in-Aid Ministry of Education, Science and Culture of Japan.
PY - 1990/2/14
Y1 - 1990/2/14
N2 - Involvement of endosomes in transport of newly synthesized acid phosphatase to lysosomes was investigated using the Golgi fraction (GF1+2), enriched in endosomes. The Golgi fraction (GF1+2) was prepared from the livers of rats given [35S]methionine and asialofetuin conjugated-horseradish peroxidase (HRP). Newly synthesized acid phosphatase in the endosomes containing internalized asialofetuin-HRP was measured as a loss of the detectable labeled enzyme after 3,3′-diaminobenzidine (DAB) and H2O2 reaction, due to formation of insoluble polymers which reduce protein antigenicity. With this procedure, acid phosphatase was all but undetectable in the Golgi fraction. Thus, newly synthesized acid phosphatase is apparently transported to lysosomes by endosomes.
AB - Involvement of endosomes in transport of newly synthesized acid phosphatase to lysosomes was investigated using the Golgi fraction (GF1+2), enriched in endosomes. The Golgi fraction (GF1+2) was prepared from the livers of rats given [35S]methionine and asialofetuin conjugated-horseradish peroxidase (HRP). Newly synthesized acid phosphatase in the endosomes containing internalized asialofetuin-HRP was measured as a loss of the detectable labeled enzyme after 3,3′-diaminobenzidine (DAB) and H2O2 reaction, due to formation of insoluble polymers which reduce protein antigenicity. With this procedure, acid phosphatase was all but undetectable in the Golgi fraction. Thus, newly synthesized acid phosphatase is apparently transported to lysosomes by endosomes.
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U2 - 10.1016/0006-291X(90)90990-5
DO - 10.1016/0006-291X(90)90990-5
M3 - Article
C2 - 1689571
AN - SCOPUS:0025019627
SN - 0006-291X
VL - 166
SP - 1176
EP - 1182
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -