Lymphocyte DNA adducts and polymorphism in the DNA repair enzyme XPD

M. Ichiba; J. Zhang; C. Kiyohara; Y. Nakanishi; K. Takayama; N. Hara; M. Enoki; K. Tomokuni

doi:10.1080/13547500010009591

Lymphocyte DNA adducts and polymorphism in the DNA repair enzyme XPD

M. Ichiba, J. Zhang, C. Kiyohara, Y. Nakanishi, K. Takayama, N. Hara, M. Enoki, K. Tomokuni

Department of Respirology

Research output: Contribution to journal › Article › peer-review

Abstract

The effect of genetic polymorphism of DNA repair enzyme on the DNA adduct levels was evaluated in this study. We explored the relationship between polymorphism in the nucleotide excision repair enzyme XPD and DNA adduct levels in lymphocytes. Lymphocyte DNA adducts were measured by a ³²P-postlabelling method from 42 subjects. XPD ex23 genotypes were identified by the PCR-RFLP method. Subjects with the XPD ex23 heterozygous genotype (AC) showed significantly lower DNA adduct levels (0.63±0.19 per 10⁸ nucleotides, n=6) than those (0.92±0.38, n=36) with major homozygous genotypes (AA). This result suggests that the genotype of the repair enzyme may be one of the important determinants for DNA adduct levels.

Original language	English
Pages (from-to)	289-293
Number of pages	5
Journal	Biomarkers
Volume	6
Issue number	4
DOIs	https://doi.org/10.1080/13547500010009591
Publication status	Published - 2001

All Science Journal Classification (ASJC) codes

Biochemistry
Clinical Biochemistry
Health, Toxicology and Mutagenesis

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title = "Lymphocyte DNA adducts and polymorphism in the DNA repair enzyme XPD",

abstract = "The effect of genetic polymorphism of DNA repair enzyme on the DNA adduct levels was evaluated in this study. We explored the relationship between polymorphism in the nucleotide excision repair enzyme XPD and DNA adduct levels in lymphocytes. Lymphocyte DNA adducts were measured by a 32P-postlabelling method from 42 subjects. XPD ex23 genotypes were identified by the PCR-RFLP method. Subjects with the XPD ex23 heterozygous genotype (AC) showed significantly lower DNA adduct levels (0.63±0.19 per 108 nucleotides, n=6) than those (0.92±0.38, n=36) with major homozygous genotypes (AA). This result suggests that the genotype of the repair enzyme may be one of the important determinants for DNA adduct levels.",

author = "M. Ichiba and J. Zhang and C. Kiyohara and Y. Nakanishi and K. Takayama and N. Hara and M. Enoki and K. Tomokuni",

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T1 - Lymphocyte DNA adducts and polymorphism in the DNA repair enzyme XPD

AU - Ichiba, M.

AU - Zhang, J.

AU - Kiyohara, C.

AU - Nakanishi, Y.

AU - Takayama, K.

AU - Hara, N.

AU - Enoki, M.

AU - Tomokuni, K.

PY - 2001

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N2 - The effect of genetic polymorphism of DNA repair enzyme on the DNA adduct levels was evaluated in this study. We explored the relationship between polymorphism in the nucleotide excision repair enzyme XPD and DNA adduct levels in lymphocytes. Lymphocyte DNA adducts were measured by a 32P-postlabelling method from 42 subjects. XPD ex23 genotypes were identified by the PCR-RFLP method. Subjects with the XPD ex23 heterozygous genotype (AC) showed significantly lower DNA adduct levels (0.63±0.19 per 108 nucleotides, n=6) than those (0.92±0.38, n=36) with major homozygous genotypes (AA). This result suggests that the genotype of the repair enzyme may be one of the important determinants for DNA adduct levels.

AB - The effect of genetic polymorphism of DNA repair enzyme on the DNA adduct levels was evaluated in this study. We explored the relationship between polymorphism in the nucleotide excision repair enzyme XPD and DNA adduct levels in lymphocytes. Lymphocyte DNA adducts were measured by a 32P-postlabelling method from 42 subjects. XPD ex23 genotypes were identified by the PCR-RFLP method. Subjects with the XPD ex23 heterozygous genotype (AC) showed significantly lower DNA adduct levels (0.63±0.19 per 108 nucleotides, n=6) than those (0.92±0.38, n=36) with major homozygous genotypes (AA). This result suggests that the genotype of the repair enzyme may be one of the important determinants for DNA adduct levels.

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Lymphocyte DNA adducts and polymorphism in the DNA repair enzyme XPD

Abstract

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