TY - JOUR
T1 - Low Mw sulfated curdlan with improved water solubility forms macromolecular complexes with polycytidylic acid
AU - Koumoto, Kazuya
AU - Umeda, Mariko
AU - Numata, Munenori
AU - Matsumoto, Takahiro
AU - Sakurai, Kazuo
AU - Kunitake, Toyoki
AU - Shinkai, Seiji
N1 - Funding Information:
We thank Taito Co., Japan for providing the schizophyllan samples. K.K. is indebted to the Japan Society for the Promotion of Science (JSPS) for financial support. This research has been conducted under the financial support from the JST SORST Program.
PY - 2004/1/2
Y1 - 2004/1/2
N2 - The water solubility of curdlan was enhanced by partial sulfation at O-6. Circular dichroism measurements suggest that the sulfated curdlan with the degree of substitution (DS) from 0 to 8.7mol% forms macromolecular complexes with polycytidylic acid (poly(C)). Although the thermal stability of the complexes decreased with increase in DS, this could be overlapped by addition of NaCl in the concentration above that of serum. The results clearly indicate that the drawback arising from the electrostatic repulsion between the anionic charges can be partially compensated by the presence of salt. Furthermore, the polynucleotide chain complexed with the sulfated curdlan was protected from the enzymatic hydrolysis, corroborating the assumption that the sulfated curdlan has an ability to bind poly(C).
AB - The water solubility of curdlan was enhanced by partial sulfation at O-6. Circular dichroism measurements suggest that the sulfated curdlan with the degree of substitution (DS) from 0 to 8.7mol% forms macromolecular complexes with polycytidylic acid (poly(C)). Although the thermal stability of the complexes decreased with increase in DS, this could be overlapped by addition of NaCl in the concentration above that of serum. The results clearly indicate that the drawback arising from the electrostatic repulsion between the anionic charges can be partially compensated by the presence of salt. Furthermore, the polynucleotide chain complexed with the sulfated curdlan was protected from the enzymatic hydrolysis, corroborating the assumption that the sulfated curdlan has an ability to bind poly(C).
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U2 - 10.1016/j.carres.2003.09.022
DO - 10.1016/j.carres.2003.09.022
M3 - Article
C2 - 14659684
AN - SCOPUS:0344628630
SN - 0008-6215
VL - 339
SP - 161
EP - 167
JO - Carbohydrate Research
JF - Carbohydrate Research
IS - 1
ER -