@inbook{40988878d187456b971019f210af22f1,
title = "Low Input Genome-Wide DNA Methylation Analysis with Minimal Library Amplification",
abstract = "Whole genome bisulfite sequencing (WGBS) is a high-throughput DNA sequencing-based technique that is used to determine genome-wide DNA methylation patterns at base resolution. Library construction by post-bisulfite adaptor tagging (PBAT) extends the application of WGBS to several hundred cells and minimizes the required number of library amplification cycles. We herein describe a PBAT protocol to prepare WGBS libraries from 200 cells and introduce the outline of a downstream bioinformatic analysis. The prepared library can typically generate 800 million sequencing reads, which is sufficient to cover the human and mouse genomes approximately 15 times, using the Illumina NovaSeq 6000 sequencing system.",
author = "{Au Yeung}, {Wan Kin} and Hiroyuki Sasaki",
note = "Funding Information: This work was supported by a JSPS KAKENHI grant to H.S. (JP18H05214). We would like to thank Dr. Fumihito Miura and Dr. Kenjiro Shirane (Kyushu University) for their helpful discussion. Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2380-0_14",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "233--250",
booktitle = "Methods in Molecular Biology",
}