Low-cost three-dimensional gait analysis system for mice with an infrared depth sensor

Akihiro Nakamura, Hiroyuki Funaya, Naohiro Uezono, Kinichi Nakashima, Yasumasa Ishida, Tomohiro Suzuki, Shigeharu Wakana, Tomohiro Shibata

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)


Three-dimensional (3D) open-field gait analysis of mice is an essential procedure in genetic and nerve regeneration research. Existing gait analysis systems are generally expensive and may interfere with the natural behaviors of mice because of optical markers and transparent floors. In contrast, the proposed system captures the subjects shape from beneath using a low-cost infrared depth sensor (Microsoft Kinect) and an opaque infrared pass filter. This means that we can track footprints and 3D paw-tip positions without optical markers or a transparent floor, thereby preventing any behavioral changes. Our experimental results suggest with healthy mice that they are more active on opaque floors and spend more time in the center of the open-field, when compared with transparent floors. The proposed system detected footprints with a comparable performance to existing systems, and precisely tracked the 3D paw-tip positions in the depth image coordinates.

Original languageEnglish
Pages (from-to)55-62
Number of pages8
JournalNeuroscience Research
Publication statusPublished - Nov 2015

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)


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