Loss of occludin affects tricellular localization of tricellulin

Junichi Ikenouchi, Hiroyuki Sasaki, Sachiko Tsukita, Mikio Furuse, Shoichiro Tsukita

Research output: Contribution to journalArticlepeer-review

160 Citations (Scopus)


The tricellular tight junction (tTJ) forms at the convergence of bicellular tight junctions (bTJs) where three epithelial cells meet in polarized epithelia, and it is required for the maintenance of the transepithelial barrier. Tricellulin is a four transmembrane domain protein recently identified as the first marker of tTJ, but little is known about how tricellulin is localized at tTJs. As for the molecular mechanism of association of tricellulin with tight junctions (TJs), we found that tricellulin was incorporated into claudin-based TJs independently of binding to zona occludens-1. Unexpectedly, exogenous expression of tricellulin increased cross-links of TJ strands in the plasma membrane. As for the molecular mechanisms for localization of tricellulin at tricellular junctions, we found that knockdown of occludin caused mislo-calization of tricellulin to bTJs, implying that occludin supports tricellular localization of tricellulin by excluding tricellulin from bTJs.

Original languageEnglish
Pages (from-to)4687-4693
Number of pages7
JournalMolecular biology of the cell
Issue number11
Publication statusPublished - Nov 2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology


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