TY - JOUR
T1 - Log P effect of organic solvents on a thermophilic alcohol dehydrogenase
AU - Hirakawa, Hidehiko
AU - Kamiya, Noriho
AU - Kawarabayashi, Yutaka
AU - Nagamune, Teruyuki
N1 - Funding Information:
We are grateful to the Department of Biotechnology, National Institute of Technology and Evaluation which kindly provided the gene of A. pernix ADH. The present work was supported partly by a Grant-in-Aid for the 21st century COE program, “Human-Friendly Material Based on Chemistry” from the Ministry of Education, Culture, Science, Sport and Technology of Japan.
PY - 2005/4/15
Y1 - 2005/4/15
N2 - An alcohol dehydrogenase from the hyperthermophilic archaeon Aeropyrum pernix was activated by water-miscible organic solvents. This activation was influenced by the kind and the concentration of the added organic solvents. The kcat was increased by a factor of over ten when the mole fraction of acetonitrile was 0.1. This effect was large when organic solvents with large log P values were added. In fact, the kcat showed a strong positive correlation with the log P value of the mixed solvent at a constant mole fraction of water, while it was not affected by the kind of organic solvents added. Both the activation enthalpy and the entropy decreased with an increase in log P. The contribution of the activation enthalpy to the free energy of activation was larger than that of the activation entropy, and the free energy of activation decreased with an increase in log P.
AB - An alcohol dehydrogenase from the hyperthermophilic archaeon Aeropyrum pernix was activated by water-miscible organic solvents. This activation was influenced by the kind and the concentration of the added organic solvents. The kcat was increased by a factor of over ten when the mole fraction of acetonitrile was 0.1. This effect was large when organic solvents with large log P values were added. In fact, the kcat showed a strong positive correlation with the log P value of the mixed solvent at a constant mole fraction of water, while it was not affected by the kind of organic solvents added. Both the activation enthalpy and the entropy decreased with an increase in log P. The contribution of the activation enthalpy to the free energy of activation was larger than that of the activation entropy, and the free energy of activation decreased with an increase in log P.
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U2 - 10.1016/j.bbapap.2004.12.007
DO - 10.1016/j.bbapap.2004.12.007
M3 - Article
C2 - 15752697
AN - SCOPUS:14744283390
SN - 1570-9639
VL - 1748
SP - 94
EP - 99
JO - Biochimica et Biophysica Acta - Proteins and Proteomics
JF - Biochimica et Biophysica Acta - Proteins and Proteomics
IS - 1
ER -