TY - JOUR
T1 - Localized gene transfer and its application for the study of central cardiovascular control
AU - Hirooka, Yoshitaka
N1 - Funding Information:
These studies were supported by Grants-in-Aid for Scientific Research (C11670689, C13670721, C15590757, C17590745) from the Japan Society for the Promotion of Science, and by a Grant for Scientific Research on the Autonomic Nervous System and Hypertension from Kimura Memorial Heart Foundation/Pfizer Pharmaceuticals. The author thanks all contributors at our institute in this series of studies. The author also thanks Drs. Donald D. Heistad, Beverly L. Davidson, and John F. Engelhardt (The University of Iowa Gene Transfer Vector Core, supported by National Institute of Health Grants and the Carver Foundation) for the preparation of vectors.
PY - 2006/6/30
Y1 - 2006/6/30
N2 - The arterial baroreceptor reflex is the major feedback control system that acts to stabilize blood pressure. Abnormalities of this reflex are considered to be an underlying mechanism in the cardiovascular diseases such as hypertension and heart failure. There is accumulating evidence, however, that central nervous system mechanisms are involved in the enhanced sympathetic drive that occurs in these disease states. This article reviews studies performed in our laboratory in which a gene transfer technique, in combination with other methods, was used to determine the functional role of the central control of cardiovascular regulation. We developed a technique to transfer adenovirus vectors encoding specific genes into the nucleus tractus solitarii (NTS) or the rostral ventral medulla (RVLM) of rats in vivo. We applied this technique to hypertensive rats as well as in mice with heart failure to explore the pathophysiological significance of nitric oxide, reactive oxygen species, and Rho-kinase.
AB - The arterial baroreceptor reflex is the major feedback control system that acts to stabilize blood pressure. Abnormalities of this reflex are considered to be an underlying mechanism in the cardiovascular diseases such as hypertension and heart failure. There is accumulating evidence, however, that central nervous system mechanisms are involved in the enhanced sympathetic drive that occurs in these disease states. This article reviews studies performed in our laboratory in which a gene transfer technique, in combination with other methods, was used to determine the functional role of the central control of cardiovascular regulation. We developed a technique to transfer adenovirus vectors encoding specific genes into the nucleus tractus solitarii (NTS) or the rostral ventral medulla (RVLM) of rats in vivo. We applied this technique to hypertensive rats as well as in mice with heart failure to explore the pathophysiological significance of nitric oxide, reactive oxygen species, and Rho-kinase.
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U2 - 10.1016/j.autneu.2006.02.017
DO - 10.1016/j.autneu.2006.02.017
M3 - Review article
C2 - 16616703
AN - SCOPUS:33744550611
SN - 1566-0702
VL - 126-127
SP - 120
EP - 129
JO - Autonomic Neuroscience: Basic and Clinical
JF - Autonomic Neuroscience: Basic and Clinical
ER -
