TY - JOUR
T1 - Localization of PTP-FERM in nerve processes through its FERM domain
AU - Uchida, Yohzo
AU - Ogata, Masato
AU - Mori, Yoshiko
AU - Oh-hora, Masatsugu
AU - Hatano, Naoya
AU - Hamaoka, Toshiyuki
N1 - Funding Information:
1This work was supported in part by a Grant-in-Aid for Science Research from the Ministry of Education, Science and Culture of Japan, and Osaka Foundation for Promotion of Clinical Immunology.
PY - 2002
Y1 - 2002
N2 - PTP-FERM is a protein tyrosine phosphatase (PTP) of Caenorhabditis elegans containing a FERM domain and a PDZ domain. Here we report the characterization of PTP-FERM and the essential role of its FERM domain in the localization of PTP-FERM in the worm. There are at least three alternatively spliced PTP-FERM isoforms, all of which contain a band 4.1/FERM domain, a PDZ domain, and a catalytic domain. PTP-FERM possessed phosphatase activity. PTP-FERM was expressed predominantly in neurons in the nerve ring and the ventral nerve cord. PTP-FERM was found in the nerve processes and to be enriched in the perimembrane region. Studies using various deletion mutants revealed that the FERM domain was essential and sufficient for the subcellular localization. These results suggest the essential role of the FERM domain in the function of PTP-FERM in the neurons of C. elegans.
AB - PTP-FERM is a protein tyrosine phosphatase (PTP) of Caenorhabditis elegans containing a FERM domain and a PDZ domain. Here we report the characterization of PTP-FERM and the essential role of its FERM domain in the localization of PTP-FERM in the worm. There are at least three alternatively spliced PTP-FERM isoforms, all of which contain a band 4.1/FERM domain, a PDZ domain, and a catalytic domain. PTP-FERM possessed phosphatase activity. PTP-FERM was expressed predominantly in neurons in the nerve ring and the ventral nerve cord. PTP-FERM was found in the nerve processes and to be enriched in the perimembrane region. Studies using various deletion mutants revealed that the FERM domain was essential and sufficient for the subcellular localization. These results suggest the essential role of the FERM domain in the function of PTP-FERM in the neurons of C. elegans.
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U2 - 10.1006/bbrc.2002.6613
DO - 10.1006/bbrc.2002.6613
M3 - Article
C2 - 11890665
AN - SCOPUS:0036290139
SN - 0006-291X
VL - 292
SP - 13
EP - 19
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -