Localization of metallothionein in nuclei of growing primary cultured adult rat hepatocytes

Kazutake Tsujikawa; Takumi Imai; Makoto Kakutani; Yuzo Kayamori; Tsutomu Mimura; Noriko Otaki; Masami Kimura; Ryuichi Fukuyama; Nobuyoshi Shimizu

doi:10.1016/0014-5793(91)80597-V

Localization of metallothionein in nuclei of growing primary cultured adult rat hepatocytes

Kazutake Tsujikawa, Takumi Imai, Makoto Kakutani, Yuzo Kayamori, Tsutomu Mimura, Noriko Otaki, Masami Kimura, Ryuichi Fukuyama, Nobuyoshi Shimizu

Research output: Contribution to journal › Article › peer-review

101 Citations (Scopus)

Abstract

In primary cultured adult rat hepatocytes stimulated by epidermal growth factor and insulin, dramatic changes in the subcellular distribution of metallothionein were clarified by indirect immunofluorescence using antisera specific for this protein. Metallothionein was detected only in the cytoplasm of cultured hepatocytes in the G₀ and G₁ phases, but was concentrated in the cell nuclei in the early S phase. The strongest staining pattern in the nuclei was observed 12 h after stimulation. Subsequently, the intensity of metallothionein staining in the nuclei decreased. These results suggests that primary cultured hepatocytes are suitable for examining the relation between subcellular localization of metallothionein and cell growth.

Original language	English
Pages (from-to)	239-242
Number of pages	4
Journal	FEBS Letters
Volume	283
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(91)80597-V
Publication status	Published - Jun 3 1991
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(91)80597-V

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title = "Localization of metallothionein in nuclei of growing primary cultured adult rat hepatocytes",

abstract = "In primary cultured adult rat hepatocytes stimulated by epidermal growth factor and insulin, dramatic changes in the subcellular distribution of metallothionein were clarified by indirect immunofluorescence using antisera specific for this protein. Metallothionein was detected only in the cytoplasm of cultured hepatocytes in the G0 and G1 phases, but was concentrated in the cell nuclei in the early S phase. The strongest staining pattern in the nuclei was observed 12 h after stimulation. Subsequently, the intensity of metallothionein staining in the nuclei decreased. These results suggests that primary cultured hepatocytes are suitable for examining the relation between subcellular localization of metallothionein and cell growth.",

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doi = "10.1016/0014-5793(91)80597-V",

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T1 - Localization of metallothionein in nuclei of growing primary cultured adult rat hepatocytes

AU - Tsujikawa, Kazutake

AU - Imai, Takumi

AU - Kakutani, Makoto

AU - Kayamori, Yuzo

AU - Mimura, Tsutomu

AU - Otaki, Noriko

AU - Kimura, Masami

AU - Fukuyama, Ryuichi

AU - Shimizu, Nobuyoshi

PY - 1991/6/3

Y1 - 1991/6/3

N2 - In primary cultured adult rat hepatocytes stimulated by epidermal growth factor and insulin, dramatic changes in the subcellular distribution of metallothionein were clarified by indirect immunofluorescence using antisera specific for this protein. Metallothionein was detected only in the cytoplasm of cultured hepatocytes in the G0 and G1 phases, but was concentrated in the cell nuclei in the early S phase. The strongest staining pattern in the nuclei was observed 12 h after stimulation. Subsequently, the intensity of metallothionein staining in the nuclei decreased. These results suggests that primary cultured hepatocytes are suitable for examining the relation between subcellular localization of metallothionein and cell growth.

AB - In primary cultured adult rat hepatocytes stimulated by epidermal growth factor and insulin, dramatic changes in the subcellular distribution of metallothionein were clarified by indirect immunofluorescence using antisera specific for this protein. Metallothionein was detected only in the cytoplasm of cultured hepatocytes in the G0 and G1 phases, but was concentrated in the cell nuclei in the early S phase. The strongest staining pattern in the nuclei was observed 12 h after stimulation. Subsequently, the intensity of metallothionein staining in the nuclei decreased. These results suggests that primary cultured hepatocytes are suitable for examining the relation between subcellular localization of metallothionein and cell growth.

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Localization of metallothionein in nuclei of growing primary cultured adult rat hepatocytes

Abstract

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