Localization of metallothionein in nuclei of growing primary cultured adult rat hepatocytes

Kazutake Tsujikawa, Takumi Imai, Makoto Kakutani, Yuzo Kayamori, Tsutomu Mimura, Noriko Otaki, Masami Kimura, Ryuichi Fukuyama, Nobuyoshi Shimizu

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101 Citations (Scopus)


In primary cultured adult rat hepatocytes stimulated by epidermal growth factor and insulin, dramatic changes in the subcellular distribution of metallothionein were clarified by indirect immunofluorescence using antisera specific for this protein. Metallothionein was detected only in the cytoplasm of cultured hepatocytes in the G0 and G1 phases, but was concentrated in the cell nuclei in the early S phase. The strongest staining pattern in the nuclei was observed 12 h after stimulation. Subsequently, the intensity of metallothionein staining in the nuclei decreased. These results suggests that primary cultured hepatocytes are suitable for examining the relation between subcellular localization of metallothionein and cell growth.

Original languageEnglish
Pages (from-to)239-242
Number of pages4
JournalFEBS Letters
Issue number2
Publication statusPublished - Jun 3 1991
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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