Local OPG gene transfer to periodontal tissue inhibits orthodontic tooth movement

H. Kanzaki, M. Chiba, I. Takahashi, N. Haruyama, M. Nishimura, H. Mitani

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132 Citations (Scopus)


Previously, we discovered that RANKL expression is induced in compressed periodontal ligament cells, and that this promotes osteoclastogenesis on the compression side in orthodontic tooth movement. We hypothesized that local OPG gene transfer to the periodontium would neutralize the RANKL activity induced by mechanical compressive force, thereby inhibiting osteoclastogenesis and diminishing tooth movement. The upper first molars of six-week-old male Wistar rats were moved palatally by means of a fixed-orthodontic wire. A mouse OPG expression plasmid [pcDNA3.1(+)-mOPG] was constructed, and the production of functional OPG protein was confirmed in vitro. The inactivated HVJ envelope vector containing pcDNA3.1(+)-mOPG or PBS was injected periodically into the palatal periodontal tissue of upper first molars. When this local OPG gene transfer was performed, OPG production was induced, and osteoclastogenesis was inhibited. Local OPG gene transfer significantly diminished tooth movement. In this study, we report that OPG gene transfer to periodontal tissue inhibited RANKL-mediated osteoclastogenesis and inhibited experimental tooth movement.

Original languageEnglish
Pages (from-to)920-925
Number of pages6
JournalJournal of Dental Research
Issue number12
Publication statusPublished - Dec 2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Dentistry(all)


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