Live-cell metabolic analyzer protocol for measuring glucose and lactate metabolic changes in human cells

Kenji Miki; Mikako Yagi; Ko Igami; Hiroki Kittaka; Akito Tani; Natsuki Horiuchi; Satoshi Fukumoto; Koji Yoshimoto; Takeshi Uchiumi

doi:10.1016/j.xpro.2024.103518

Live-cell metabolic analyzer protocol for measuring glucose and lactate metabolic changes in human cells

Kenji Miki
, Mikako Yagi
, Ko Igami
, Hiroki Kittaka
, Akito Tani
, Natsuki Horiuchi
, Satoshi Fukumoto
, Koji Yoshimoto
, Takeshi Uchiumi

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Understanding metabolic conditions related to glycolysis dependence is crucial for developing new treatments in cancer and regenerative medicine. This protocol details a method for using the live-cell metabolic analyzer (LiCellMo) to measure continuous changes in glucose consumption and lactate production in cultured human cells. LiCellMo provides real-time data on consecutive metabolic changes, improving measurements of these processes in various contexts, including in cancer and regenerative treatments.

Original language	English
Article number	103518
Journal	STAR Protocols
Volume	6
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2024.103518
Publication status	Published - Mar 21 2025

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

General Neuroscience
General Immunology and Microbiology
General Biochemistry,Genetics and Molecular Biology

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10.1016/j.xpro.2024.103518

Cite this

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title = "Live-cell metabolic analyzer protocol for measuring glucose and lactate metabolic changes in human cells",

abstract = "Understanding metabolic conditions related to glycolysis dependence is crucial for developing new treatments in cancer and regenerative medicine. This protocol details a method for using the live-cell metabolic analyzer (LiCellMo) to measure continuous changes in glucose consumption and lactate production in cultured human cells. LiCellMo provides real-time data on consecutive metabolic changes, improving measurements of these processes in various contexts, including in cancer and regenerative treatments.",

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author = "Kenji Miki and Mikako Yagi and Ko Igami and Hiroki Kittaka and Akito Tani and Natsuki Horiuchi and Satoshi Fukumoto and Koji Yoshimoto and Takeshi Uchiumi",

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doi = "10.1016/j.xpro.2024.103518",

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T1 - Live-cell metabolic analyzer protocol for measuring glucose and lactate metabolic changes in human cells

AU - Miki, Kenji

AU - Yagi, Mikako

AU - Igami, Ko

AU - Kittaka, Hiroki

AU - Tani, Akito

AU - Horiuchi, Natsuki

AU - Fukumoto, Satoshi

AU - Yoshimoto, Koji

AU - Uchiumi, Takeshi

PY - 2025/3/21

Y1 - 2025/3/21

N2 - Understanding metabolic conditions related to glycolysis dependence is crucial for developing new treatments in cancer and regenerative medicine. This protocol details a method for using the live-cell metabolic analyzer (LiCellMo) to measure continuous changes in glucose consumption and lactate production in cultured human cells. LiCellMo provides real-time data on consecutive metabolic changes, improving measurements of these processes in various contexts, including in cancer and regenerative treatments.

AB - Understanding metabolic conditions related to glycolysis dependence is crucial for developing new treatments in cancer and regenerative medicine. This protocol details a method for using the live-cell metabolic analyzer (LiCellMo) to measure continuous changes in glucose consumption and lactate production in cultured human cells. LiCellMo provides real-time data on consecutive metabolic changes, improving measurements of these processes in various contexts, including in cancer and regenerative treatments.

KW - cell biology

KW - cell culture

KW - metabolism

UR - https://www.scopus.com/pages/publications/85214488334

UR - https://www.scopus.com/pages/publications/85214488334#tab=citedBy

U2 - 10.1016/j.xpro.2024.103518

DO - 10.1016/j.xpro.2024.103518

M3 - Article

C2 - 39799573

AN - SCOPUS:85214488334

SN - 2666-1667

VL - 6

JO - STAR Protocols

JF - STAR Protocols

IS - 1

M1 - 103518

ER -

Live-cell metabolic analyzer protocol for measuring glucose and lactate metabolic changes in human cells

Abstract

UN SDGs

All Science Journal Classification (ASJC) codes

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