Liquid-liquid extraction of enzymatically synthesized functional RNA oligonucleotides using reverse micelles with a DNA-surfactant

Tatsuo Maruyama; Naoki Ishizu; Yuka Eguchi; Takuya Hosogi; Masahiro Goto

doi:10.1039/c6cc06985a

Liquid-liquid extraction of enzymatically synthesized functional RNA oligonucleotides using reverse micelles with a DNA-surfactant

Tatsuo Maruyama, Naoki Ishizu, Yuka Eguchi, Takuya Hosogi, Masahiro Goto

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

We successfully implemented solvent extraction of short, single-stranded RNA using reverse micelles (water-in-oil microemulsions) with a DNA-surfactant. A thrombin-binding RNA aptamer was enzymatically synthesized and purified by extraction using the reverse micellar system. The extracted RNA aptamer retained thrombin-binding activity after the extraction procedure.

Original language	English
Pages (from-to)	12376-12379
Number of pages	4
Journal	Chemical Communications
Volume	52
Issue number	83
DOIs	https://doi.org/10.1039/c6cc06985a
Publication status	Published - 2016

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Ceramics and Composites
Metals and Alloys
Materials Chemistry
Surfaces, Coatings and Films
Chemistry(all)
Catalysis

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10.1039/c6cc06985a

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title = "Liquid-liquid extraction of enzymatically synthesized functional RNA oligonucleotides using reverse micelles with a DNA-surfactant",

abstract = "We successfully implemented solvent extraction of short, single-stranded RNA using reverse micelles (water-in-oil microemulsions) with a DNA-surfactant. A thrombin-binding RNA aptamer was enzymatically synthesized and purified by extraction using the reverse micellar system. The extracted RNA aptamer retained thrombin-binding activity after the extraction procedure.",

author = "Tatsuo Maruyama and Naoki Ishizu and Yuka Eguchi and Takuya Hosogi and Masahiro Goto",

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AU - Maruyama, Tatsuo

AU - Ishizu, Naoki

AU - Eguchi, Yuka

AU - Hosogi, Takuya

AU - Goto, Masahiro

PY - 2016

Y1 - 2016

N2 - We successfully implemented solvent extraction of short, single-stranded RNA using reverse micelles (water-in-oil microemulsions) with a DNA-surfactant. A thrombin-binding RNA aptamer was enzymatically synthesized and purified by extraction using the reverse micellar system. The extracted RNA aptamer retained thrombin-binding activity after the extraction procedure.

AB - We successfully implemented solvent extraction of short, single-stranded RNA using reverse micelles (water-in-oil microemulsions) with a DNA-surfactant. A thrombin-binding RNA aptamer was enzymatically synthesized and purified by extraction using the reverse micellar system. The extracted RNA aptamer retained thrombin-binding activity after the extraction procedure.

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JO - Chemical Communications

JF - Chemical Communications

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ER -

Liquid-liquid extraction of enzymatically synthesized functional RNA oligonucleotides using reverse micelles with a DNA-surfactant

Abstract

All Science Journal Classification (ASJC) codes

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