TY - CHAP
T1 - Liposome deformation by imbalance of pH and ionic strength across the membrane
AU - Kuroda, Osami
AU - Seto, Hiroshige
AU - Narita, Takayuki
AU - Yamanaka, Michio
AU - Oishi, Yushi
N1 - Funding Information:
The authors want to thank Dr. Michael Ibele for pointing out mistakes and many valuable discussions of the manuscript. This work was supported by JSPS Research Fellowships for Young Scientists and by Grants-in-Aid for Scientific (No. 21750221).
PY - 2010
Y1 - 2010
N2 - The deformation of giant dioleoylphosphatidylcholine (DOPC) liposomes in solution and the properties of DOPC monolayers were investigated by florescence microscopy and surface pressure-area (π-A) isotherm measurements, respectively. These measurements were taken as functions of pH and ionic strength. When the ionic strength was changed from 0.001 to 0.6 at constant pH of 5.6, the coverage of the DOPC monolayer expanded by 10%, and the liposomes formed small protrusions. When the pH was changed from 5.6 to 3.5 at a constant ionic strength of 0.001, the monolayer coverage shrank by 10%. During this process the external liposome morphology remained the same, but new, smaller vesicles appeared within the interior of the liposomes. Simultaneously changing the pH and the ionic strength to their final values (3.5 and 0.6, respectively), resulted in an expanded monolayer and produced long, protruded liposomes. Our results suggest that the deformation of liposomes is not only driven by osmotic pressure but also the condensed states in each monolayers composing liposome membrane.
AB - The deformation of giant dioleoylphosphatidylcholine (DOPC) liposomes in solution and the properties of DOPC monolayers were investigated by florescence microscopy and surface pressure-area (π-A) isotherm measurements, respectively. These measurements were taken as functions of pH and ionic strength. When the ionic strength was changed from 0.001 to 0.6 at constant pH of 5.6, the coverage of the DOPC monolayer expanded by 10%, and the liposomes formed small protrusions. When the pH was changed from 5.6 to 3.5 at a constant ionic strength of 0.001, the monolayer coverage shrank by 10%. During this process the external liposome morphology remained the same, but new, smaller vesicles appeared within the interior of the liposomes. Simultaneously changing the pH and the ionic strength to their final values (3.5 and 0.6, respectively), resulted in an expanded monolayer and produced long, protruded liposomes. Our results suggest that the deformation of liposomes is not only driven by osmotic pressure but also the condensed states in each monolayers composing liposome membrane.
UR - http://www.scopus.com/inward/record.url?scp=80051638154&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80051638154&partnerID=8YFLogxK
U2 - 10.1007/978-3-642-19038-4_9
DO - 10.1007/978-3-642-19038-4_9
M3 - Chapter
AN - SCOPUS:80051638154
SN - 9783642190377
T3 - Progress in Colloid and Polymer Science
SP - 49
EP - 54
BT - Trends in Colloid and Interface Science XXIV
A2 - Starov, Victor
A2 - Prochazka, Karel
ER -