Lipoprotein profiling methodology based on determination of apolipoprotein concentration

Hiroaki Takeda; Yoshihiro Izumi; Atsumi Tomita; Tomonari Koike; Masashi Shiomi; Eiichiro Fukusaki; Fumio Matsuda; Takeshi Bamba

doi:10.4155/bio-2016-0234

Lipoprotein profiling methodology based on determination of apolipoprotein concentration

Hiroaki Takeda, Yoshihiro Izumi, Atsumi Tomita, Tomonari Koike, Masashi Shiomi, Eiichiro Fukusaki, Fumio Matsuda, Takeshi Bamba

Research output: Contribution to journal › Article › peer-review

Abstract

Aim: Abnormal lipid metabolism results in the alteration of lipid compositions in lipoproteins; therefore an accurate and quantitative analytical approach is required for the detailed structural characterization of lipoproteins. However, the specific lipid composition of each lipoprotein particle is poorly understood. Materials & methods: Lipid composition of very-low-density lipoprotein and low-density lipoprotein particles derived from myocardial infarction-prone rabbits was determined by normalization of lipidomics data using apoB-100 levels. Results: The ratio of lipid levels between very-low-density lipoprotein and low-density lipoprotein particles was different according to not only lipid classes, but also phosphatidylethanolamine subclasses by applying our developed methodology to myocardial infarction-prone rabbits. Conclusion: Our novel analytical approach represents to be a potentially useful tool to obtain particle-specific lipid components of lipoproteins.

Original language	English
Pages (from-to)	9-19
Number of pages	11
Journal	Bioanalysis
Volume	9
Issue number	1
DOIs	https://doi.org/10.4155/bio-2016-0234
Publication status	Published - Jan 2017

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Pharmacology, Toxicology and Pharmaceutics(all)
Clinical Biochemistry
Medical Laboratory Technology

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title = "Lipoprotein profiling methodology based on determination of apolipoprotein concentration",

abstract = "Aim: Abnormal lipid metabolism results in the alteration of lipid compositions in lipoproteins; therefore an accurate and quantitative analytical approach is required for the detailed structural characterization of lipoproteins. However, the specific lipid composition of each lipoprotein particle is poorly understood. Materials & methods: Lipid composition of very-low-density lipoprotein and low-density lipoprotein particles derived from myocardial infarction-prone rabbits was determined by normalization of lipidomics data using apoB-100 levels. Results: The ratio of lipid levels between very-low-density lipoprotein and low-density lipoprotein particles was different according to not only lipid classes, but also phosphatidylethanolamine subclasses by applying our developed methodology to myocardial infarction-prone rabbits. Conclusion: Our novel analytical approach represents to be a potentially useful tool to obtain particle-specific lipid components of lipoproteins.",

author = "Hiroaki Takeda and Yoshihiro Izumi and Atsumi Tomita and Tomonari Koike and Masashi Shiomi and Eiichiro Fukusaki and Fumio Matsuda and Takeshi Bamba",

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T1 - Lipoprotein profiling methodology based on determination of apolipoprotein concentration

AU - Takeda, Hiroaki

AU - Izumi, Yoshihiro

AU - Tomita, Atsumi

AU - Koike, Tomonari

AU - Shiomi, Masashi

AU - Fukusaki, Eiichiro

AU - Matsuda, Fumio

AU - Bamba, Takeshi

PY - 2017/1

Y1 - 2017/1

N2 - Aim: Abnormal lipid metabolism results in the alteration of lipid compositions in lipoproteins; therefore an accurate and quantitative analytical approach is required for the detailed structural characterization of lipoproteins. However, the specific lipid composition of each lipoprotein particle is poorly understood. Materials & methods: Lipid composition of very-low-density lipoprotein and low-density lipoprotein particles derived from myocardial infarction-prone rabbits was determined by normalization of lipidomics data using apoB-100 levels. Results: The ratio of lipid levels between very-low-density lipoprotein and low-density lipoprotein particles was different according to not only lipid classes, but also phosphatidylethanolamine subclasses by applying our developed methodology to myocardial infarction-prone rabbits. Conclusion: Our novel analytical approach represents to be a potentially useful tool to obtain particle-specific lipid components of lipoproteins.

AB - Aim: Abnormal lipid metabolism results in the alteration of lipid compositions in lipoproteins; therefore an accurate and quantitative analytical approach is required for the detailed structural characterization of lipoproteins. However, the specific lipid composition of each lipoprotein particle is poorly understood. Materials & methods: Lipid composition of very-low-density lipoprotein and low-density lipoprotein particles derived from myocardial infarction-prone rabbits was determined by normalization of lipidomics data using apoB-100 levels. Results: The ratio of lipid levels between very-low-density lipoprotein and low-density lipoprotein particles was different according to not only lipid classes, but also phosphatidylethanolamine subclasses by applying our developed methodology to myocardial infarction-prone rabbits. Conclusion: Our novel analytical approach represents to be a potentially useful tool to obtain particle-specific lipid components of lipoproteins.

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Lipoprotein profiling methodology based on determination of apolipoprotein concentration

Abstract

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