Lipopolysaccharide-Induced Monocytic Cell Death for the Diagnosis of Mild Neonatal-Onset Multisystem Inflammatory Disease

Hidetoshi Takada; Masataka Ishimura; Hiroko Inada; Shouichi Ohga; Koichi Kusuhara; Yoichi Moroi; Masutaka Furue; Toshiro Hara

doi:10.1016/j.jpeds.2008.01.038

Lipopolysaccharide-Induced Monocytic Cell Death for the Diagnosis of Mild Neonatal-Onset Multisystem Inflammatory Disease

Hidetoshi Takada, Masataka Ishimura, Hiroko Inada, Shouichi Ohga, Koichi Kusuhara, Yoichi Moroi, Masutaka Furue, Toshiro Hara

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

In this report, we describe a boy who showed mild symptoms of neonatal-onset multisystem inflammatory disease. Although his symptoms and laboratory findings were similar to those of systemic juvenile idiopathic arthritis, further examinations revealed papilledema, meningitis, and a NLRP3 mutation. His peripheral blood monocytes died within 24 hours after lipopolysaccharide stimulation, a test that may be useful for diagnosis even in mild cases.

Original language	English
Pages (from-to)	885-887.e1
Journal	Journal of Pediatrics
Volume	152
Issue number	6
DOIs	https://doi.org/10.1016/j.jpeds.2008.01.038
Publication status	Published - Jun 2008

All Science Journal Classification (ASJC) codes

Pediatrics, Perinatology, and Child Health

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10.1016/j.jpeds.2008.01.038

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title = "Lipopolysaccharide-Induced Monocytic Cell Death for the Diagnosis of Mild Neonatal-Onset Multisystem Inflammatory Disease",

abstract = "In this report, we describe a boy who showed mild symptoms of neonatal-onset multisystem inflammatory disease. Although his symptoms and laboratory findings were similar to those of systemic juvenile idiopathic arthritis, further examinations revealed papilledema, meningitis, and a NLRP3 mutation. His peripheral blood monocytes died within 24 hours after lipopolysaccharide stimulation, a test that may be useful for diagnosis even in mild cases.",

author = "Hidetoshi Takada and Masataka Ishimura and Hiroko Inada and Shouichi Ohga and Koichi Kusuhara and Yoichi Moroi and Masutaka Furue and Toshiro Hara",

note = "Funding Information: All coding regions and intronic flanking sequences of the NLRP3 gene were amplified by PCR 2 and sequenced using ABI PRISM 3100 Genetic Analyzer (Perkin-Elmer; Foster City, CA). Peripheral blood mononuclear cells were incubated 24 hours in the presence or absence of LPS (0.1 μg/mL) (Sigma-Aldrich; St Louis, MO). The cells were stained with PE-cyanin 5·1 (PC5)-conjugated anti-CD14 monoclonal antibody (Beckman Coulter; Miami, FL), and the percentage of monocytes was analyzed by flow cytometer (EPICS XL, Beckman Coulter). This study was approved by the Regional Committee of Ethics for Human Research at the Faculty of Medicine of Kyushu University. ",

year = "2008",

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doi = "10.1016/j.jpeds.2008.01.038",

language = "English",

volume = "152",

pages = "885--887.e1",

journal = "Journal of Pediatrics",

issn = "0022-3476",

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T1 - Lipopolysaccharide-Induced Monocytic Cell Death for the Diagnosis of Mild Neonatal-Onset Multisystem Inflammatory Disease

AU - Takada, Hidetoshi

AU - Ishimura, Masataka

AU - Inada, Hiroko

AU - Ohga, Shouichi

AU - Kusuhara, Koichi

AU - Moroi, Yoichi

AU - Furue, Masutaka

AU - Hara, Toshiro

N1 - Funding Information: All coding regions and intronic flanking sequences of the NLRP3 gene were amplified by PCR 2 and sequenced using ABI PRISM 3100 Genetic Analyzer (Perkin-Elmer; Foster City, CA). Peripheral blood mononuclear cells were incubated 24 hours in the presence or absence of LPS (0.1 μg/mL) (Sigma-Aldrich; St Louis, MO). The cells were stained with PE-cyanin 5·1 (PC5)-conjugated anti-CD14 monoclonal antibody (Beckman Coulter; Miami, FL), and the percentage of monocytes was analyzed by flow cytometer (EPICS XL, Beckman Coulter). This study was approved by the Regional Committee of Ethics for Human Research at the Faculty of Medicine of Kyushu University.

PY - 2008/6

Y1 - 2008/6

N2 - In this report, we describe a boy who showed mild symptoms of neonatal-onset multisystem inflammatory disease. Although his symptoms and laboratory findings were similar to those of systemic juvenile idiopathic arthritis, further examinations revealed papilledema, meningitis, and a NLRP3 mutation. His peripheral blood monocytes died within 24 hours after lipopolysaccharide stimulation, a test that may be useful for diagnosis even in mild cases.

AB - In this report, we describe a boy who showed mild symptoms of neonatal-onset multisystem inflammatory disease. Although his symptoms and laboratory findings were similar to those of systemic juvenile idiopathic arthritis, further examinations revealed papilledema, meningitis, and a NLRP3 mutation. His peripheral blood monocytes died within 24 hours after lipopolysaccharide stimulation, a test that may be useful for diagnosis even in mild cases.

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Lipopolysaccharide-Induced Monocytic Cell Death for the Diagnosis of Mild Neonatal-Onset Multisystem Inflammatory Disease

Abstract

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