Lipid profiling by supercritical fluid chromatography/mass spectrometry

Takayuki Yamada, Takeshi Bamba

    Research output: Chapter in Book/Report/Conference proceedingChapter

    9 Citations (Scopus)

    Abstract

    Supercritical fluid chromatography (SFC) is a promising separation technique for comprehensive lipid profiling, or lipidomics. SFC facilitates superior high-throughput separation as compared to liquid chromatography (LC), and can be applied for the simultaneous profiling of diverse lipids with a wide range of polarities due to the physicochemical properties of its mobile phase. To date, analytical methods for fatty acyl, glycerolipid, glycerophospholipid, sphingolipid, sterol lipid, and prenol lipid profiling using SFC coupled to mass spectrometry (MS) have been developed. Hundreds of different molecular species have been identified from biological samples by combining SFC/MS and bioinformatics tools. In addition, SFC can be connected directly with supercritical fluid extraction (SFE), which is suitable for the efficient recovery of hydrophobic compounds that are susceptible to photolytic degradation and oxidation. Online SFE-SFC/MS can be utilized for the high-throughput analysis of the redox status of coenzyme Q10 (CoQ10), and phospholipids in dried plasma spots (DPS). These techniques have potential applications in the high-throughput screening of a large number of samples, in biomarker discovery, and in the elucidation of lipid metabolism mechanisms based on the functions of individual lipid molecular species.

    Original languageEnglish
    Title of host publicationNeuromethods
    PublisherHumana Press Inc.
    Pages109-131
    Number of pages23
    DOIs
    Publication statusPublished - Mar 1 2017

    Publication series

    NameNeuromethods
    Volume125
    ISSN (Print)0893-2336
    ISSN (Electronic)1940-6045

    All Science Journal Classification (ASJC) codes

    • General Neuroscience
    • General Biochemistry,Genetics and Molecular Biology
    • Pharmacology, Toxicology and Pharmaceutics(all)
    • Psychiatry and Mental health

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