Lessons from peroxisome-deficient Chinese hamster ovary (CHO) cell mutants

Yukio Fujiki, Kanji Okumoto, Naohiko Kinoshita, Kamran Ghaedi

Research output: Contribution to journalReview articlepeer-review

46 Citations (Scopus)


Cells with a genetic defect affecting a biological activity and/or a cell phenotype are generally called "cell mutants" and are a highly useful tool in genetic, biochemical, as well as cell biological research. To investigate peroxisome biogenesis and human peroxisome biogenesis disorders, more than a dozen complementation groups of Chinese hamster ovary (CHO) cell mutants defective in peroxisome assembly have been successfully isolated and established as a model system. Moreover, successful PEX gene cloning studies by taking advantage of rapid functional complementation assay of CHO cell mutants invaluably contributed to the accomplishment of isolation of pathogenic genes responsible for peroxisome biogenesis diseases. Molecular mechanisms of peroxisome assembly are currently investigated by making use of such mammalian cell mutants.

Original languageEnglish
Pages (from-to)1374-1381
Number of pages8
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Issue number12
Publication statusPublished - Dec 2006

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology


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