Leptin is an important endocrine player that directly activates gonadotropic cells in teleost fish, chub mackerel

Hirofumi Ohga, Kosuke Ito, Kohei Kakino, Hiroaki Mon, Takahiro Kusakabe, Jae Man Lee, Michiya Matsuyama

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Leptin, secreted by adipocytes, directly influences the onset of puberty in mammals. Our previous study showed that leptin stimulation could promote the secretion of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) from pituitary cells in primary culture and ovarian development in chub mackerel. This study aimed to elucidate the detailed mechanism of leptininduced effects on gonadotropin hormone-producing cells. We produced recombinant leptin using silkworm pupae and investigated the effects of leptin on FSH and LH secretion and gene expression in the primary culture of pituitary cells from chub mackerel. The presence or absence of co-expression of lepr mRNA, FSH and LH b-subunit mRNA in gonadotropic cells was examined by double-labeled in situ hybridization. The addition of leptin significantly increased the secretion and gene expression of FSH and LH from male and female pituitary cells in primary culture. In contrast, gonadotropinreleasing hormone 1 affected neither FSH secretion in cells from females nor fshb and lhb expression in cells from males and females. The expression of lepr was observed in FSH-and LH-producing cells of both males and females. The results indicate that leptin directly regulates gonadotropin synthesis and secretion and plays an important role in the induction of puberty in teleost fish.

Original languageEnglish
Article number3505
JournalCells
Volume10
Issue number12
DOIs
Publication statusPublished - Dec 2021

All Science Journal Classification (ASJC) codes

  • General Medicine

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