TY - CHAP
T1 - Lentiviral transduction of cultured microglia
AU - Masuda, Takahiro
AU - Tsuda, Makoto
AU - Tozaki-Saitoh, Hidetoshi
AU - Inoue, Kazuhide
PY - 2013
Y1 - 2013
N2 - Microglial cells are the resident immune-related glial cells of the central nervous system (CNS) that are crucial for maintaining homeostasis and sensing pathological alterations in the nervous system. To improve our understanding of the biological function of microglia, gene-transfer techniques have been improved and become widely used over the past several years. Here, we describe lentiviral-mediated transduction as a valuable tool for transduction of cultured microglial cells.
AB - Microglial cells are the resident immune-related glial cells of the central nervous system (CNS) that are crucial for maintaining homeostasis and sensing pathological alterations in the nervous system. To improve our understanding of the biological function of microglia, gene-transfer techniques have been improved and become widely used over the past several years. Here, we describe lentiviral-mediated transduction as a valuable tool for transduction of cultured microglial cells.
UR - http://www.scopus.com/inward/record.url?scp=84880123089&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84880123089&partnerID=8YFLogxK
U2 - 10.1007/978-1-62703-520-0_8
DO - 10.1007/978-1-62703-520-0_8
M3 - Chapter
C2 - 23813370
AN - SCOPUS:84880123089
SN - 9781627035194
T3 - Methods in Molecular Biology
SP - 63
EP - 67
BT - Microglia
ER -