TY - JOUR
T1 - LDL cholesterol performance of beta quantification reference measurement procedure
AU - Nakamura, Masakazu
AU - Kayamori, Yuzo
AU - Iso, Hiroyasu
AU - Kitamura, Akihiko
AU - Kiyama, Masahiko
AU - Koyama, Isao
AU - Nishimura, Kunihiro
AU - Nakai, Michikazu
AU - Noda, Hiroyuki
AU - Dasti, Mahnaz
AU - Vesper, Hubert W.
AU - Miyamoto, Yoshihiro
PY - 2014/4/20
Y1 - 2014/4/20
N2 - Background: Accurate measurement of blood lipids is crucial in cardiovascular disease risk management. The Centers for Disease Control and Prevention (CDC) Cholesterol Reference Method Laboratory Network (CRMLN) has assured the accuracy of these measurements for. over 20. years using beta quantification (BQ) method as reference measurement procedure (RMP) for high- and low-density lipoprotein cholesterol (HDL-C, LDL-C). Only limited data exist about the performance of the BQ RMP. Methods: Bottom fraction cholesterol (BFC), HDL-C, and LDL-C results after ultracentrifugation from the CDC lipid reference laboratory and the Japanese CRMLN laboratory were compared using 280 serum samples measured over the past 15. years. Data were compared statistically using method comparison and bias estimation analysis. Results: Regression analysis between CDC (x) and Osaka (y) for BFC, HDL-C, and LDL-C were y=0.988x+1.794 (R2=0.997), y=0.980x+1.118 (R2=0.994), and y=0.987x+1.200 (R2=0.997), respectively. The Osaka laboratory met performance goals for 90% to 95% of the CDC reference values. Conclusions: The BQ method by the Osaka CRMLN laboratory is highly accurate and has been stable for over 15. years. Accurate measurement of BFC is critical for the determination of LDL-C.
AB - Background: Accurate measurement of blood lipids is crucial in cardiovascular disease risk management. The Centers for Disease Control and Prevention (CDC) Cholesterol Reference Method Laboratory Network (CRMLN) has assured the accuracy of these measurements for. over 20. years using beta quantification (BQ) method as reference measurement procedure (RMP) for high- and low-density lipoprotein cholesterol (HDL-C, LDL-C). Only limited data exist about the performance of the BQ RMP. Methods: Bottom fraction cholesterol (BFC), HDL-C, and LDL-C results after ultracentrifugation from the CDC lipid reference laboratory and the Japanese CRMLN laboratory were compared using 280 serum samples measured over the past 15. years. Data were compared statistically using method comparison and bias estimation analysis. Results: Regression analysis between CDC (x) and Osaka (y) for BFC, HDL-C, and LDL-C were y=0.988x+1.794 (R2=0.997), y=0.980x+1.118 (R2=0.994), and y=0.987x+1.200 (R2=0.997), respectively. The Osaka laboratory met performance goals for 90% to 95% of the CDC reference values. Conclusions: The BQ method by the Osaka CRMLN laboratory is highly accurate and has been stable for over 15. years. Accurate measurement of BFC is critical for the determination of LDL-C.
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U2 - 10.1016/j.cca.2014.02.018
DO - 10.1016/j.cca.2014.02.018
M3 - Article
C2 - 24589772
AN - SCOPUS:84896051905
SN - 0009-8981
VL - 431
SP - 288
EP - 293
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
ER -
