TY - JOUR
T1 - Laccase-mediated degradation and reduction of toxicity of the postharvest fungicide imazalil
AU - Maruyama, Tatsuo
AU - Komatsu, Chiho
AU - Michizoe, Junji
AU - Sakai, Shinji
AU - Goto, Masahiro
N1 - Funding Information:
This research was supported by a Grant-in-Aid for the 21st Century COE Program, “Functional Innovation of Molecular Informatics” from the Ministry of Education, Culture, Science, Sports and Technology of Japan. We thank Daiwa Kasei Industry Co. Ltd. for providing laccase.
PY - 2007/3
Y1 - 2007/3
N2 - The degradation of the postharvest fungicide imazalil by the oxidative enzyme laccase was investigated in the presence of a reaction mediator (a laccase/mediator system). Of various natural mediators tested, imazalil was degraded most effectively in the presence of 4-hydroxybenzoic acid while the absence of a mediator resulted in no degradation. In this system, the mediator compound was oxidized by laccase and then the oxidized mediator oxidized imazalil. The study on the cytotoxicity of the degraded-imazalil solution against mouse fibroblast L929 cells revealed toxicity of 4-hydroxybenzoic acid. The cytotoxic study also suggested that instead of 4-hydroxybenzoic acid, the used of methyl 4-hydroxybenzoate as the reaction mediator reduced the intrinsic cytotoxicity of imazalil.
AB - The degradation of the postharvest fungicide imazalil by the oxidative enzyme laccase was investigated in the presence of a reaction mediator (a laccase/mediator system). Of various natural mediators tested, imazalil was degraded most effectively in the presence of 4-hydroxybenzoic acid while the absence of a mediator resulted in no degradation. In this system, the mediator compound was oxidized by laccase and then the oxidized mediator oxidized imazalil. The study on the cytotoxicity of the degraded-imazalil solution against mouse fibroblast L929 cells revealed toxicity of 4-hydroxybenzoic acid. The cytotoxic study also suggested that instead of 4-hydroxybenzoic acid, the used of methyl 4-hydroxybenzoate as the reaction mediator reduced the intrinsic cytotoxicity of imazalil.
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U2 - 10.1016/j.procbio.2006.09.011
DO - 10.1016/j.procbio.2006.09.011
M3 - Article
AN - SCOPUS:33847264014
SN - 1359-5113
VL - 42
SP - 459
EP - 461
JO - Process Biochemistry
JF - Process Biochemistry
IS - 3
ER -