Label-free detection of antigen protein using a metal mesh device surface-modified by an antibody

Hirokazu Seto; Seiji Kamba; Takashi Kondo; Yuichi Ogawa; Yu Hoshino; Yoshiko Miura

doi:10.2116/analsci.31.173

Label-free detection of antigen protein using a metal mesh device surface-modified by an antibody

Hirokazu Seto, Seiji Kamba, Takashi Kondo, Yuichi Ogawa, Yu Hoshino, Yoshiko Miura

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

A biosensor for protein detection was developed using antibody-immobilized metal mesh devices. Antihemoglobin antibodies were covalently immobilized on a metal mesh device. Extraordinary transmission with a dipped structure was observed for a metal mesh device immobilized with antihemoglobin antibodies as well as for the original metal mesh device. Hemoglobin in the mixture solution containing albumin at a hundred-fold concentration was detectable using antihemoglobin-immobilized MMDs. The detectability using the antihemoglobin-immobilized metal mesh device was similar to that of a commercially-available kit for the qualitative determination of hemoglobin.

Original language	English
Pages (from-to)	173-176
Number of pages	4
Journal	analytical sciences
Volume	31
Issue number	3
DOIs	https://doi.org/10.2116/analsci.31.173
Publication status	Published - 2015

All Science Journal Classification (ASJC) codes

Analytical Chemistry

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10.2116/analsci.31.173

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title = "Label-free detection of antigen protein using a metal mesh device surface-modified by an antibody",

abstract = "A biosensor for protein detection was developed using antibody-immobilized metal mesh devices. Antihemoglobin antibodies were covalently immobilized on a metal mesh device. Extraordinary transmission with a dipped structure was observed for a metal mesh device immobilized with antihemoglobin antibodies as well as for the original metal mesh device. Hemoglobin in the mixture solution containing albumin at a hundred-fold concentration was detectable using antihemoglobin-immobilized MMDs. The detectability using the antihemoglobin-immobilized metal mesh device was similar to that of a commercially-available kit for the qualitative determination of hemoglobin.",

author = "Hirokazu Seto and Seiji Kamba and Takashi Kondo and Yuichi Ogawa and Yu Hoshino and Yoshiko Miura",

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AU - Seto, Hirokazu

AU - Kamba, Seiji

AU - Kondo, Takashi

AU - Ogawa, Yuichi

AU - Hoshino, Yu

AU - Miura, Yoshiko

N1 - Publisher Copyright: © The Japan Society for Analytical Chemistry.

PY - 2015

Y1 - 2015

N2 - A biosensor for protein detection was developed using antibody-immobilized metal mesh devices. Antihemoglobin antibodies were covalently immobilized on a metal mesh device. Extraordinary transmission with a dipped structure was observed for a metal mesh device immobilized with antihemoglobin antibodies as well as for the original metal mesh device. Hemoglobin in the mixture solution containing albumin at a hundred-fold concentration was detectable using antihemoglobin-immobilized MMDs. The detectability using the antihemoglobin-immobilized metal mesh device was similar to that of a commercially-available kit for the qualitative determination of hemoglobin.

AB - A biosensor for protein detection was developed using antibody-immobilized metal mesh devices. Antihemoglobin antibodies were covalently immobilized on a metal mesh device. Extraordinary transmission with a dipped structure was observed for a metal mesh device immobilized with antihemoglobin antibodies as well as for the original metal mesh device. Hemoglobin in the mixture solution containing albumin at a hundred-fold concentration was detectable using antihemoglobin-immobilized MMDs. The detectability using the antihemoglobin-immobilized metal mesh device was similar to that of a commercially-available kit for the qualitative determination of hemoglobin.

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Label-free detection of antigen protein using a metal mesh device surface-modified by an antibody

Abstract

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