TY - JOUR
T1 - Kwakhurin-magnetic particles conjugates enable fast enzyme immunoassay for the detection of kwakhurin in Pueraria candollei
AU - Sakamoto, Seiichi
AU - Eto, Ryota
AU - Nuntawong, Poomraphie
AU - Yusakul, Gorawit
AU - Juengwatanatrakul, Thaweesak
AU - Putalun, Waraporn
AU - Fujii, Shunsuke
AU - Tanaka, Hiroyuki
AU - Morimoto, Satoshi
N1 - Funding Information:
The research in this article was supported by Grant‐in‐Aid from Development of Safety Evaluation Method of Health Food Containing , Research on Food Safety, the Health and Labour Sciences Research Grants, the Ministry of Health, Labour and Welfare, Japan. P. mirifica
Publisher Copyright:
© 2020 John Wiley & Sons, Ltd.
PY - 2020/11/1
Y1 - 2020/11/1
N2 - Introduction: Kwakhurin (Kwa) is one of the unique isoflavonoids produced in Pueraria candollei var. mirifica (P. candollei), which has long been used as folk medicine for rejuvenation in Thailand. Recently, the use of P. candollei-derived products has widely spread among Japanese women for cosmetic purposes. Correspondingly, there has been an increase in the number of reports regarding possible health hazards caused by estrogenic activity inherent to the plant; thus, the need for a detailed evaluation of the phytoestrogen content of P. candollei-derived products has gained a sense of urgency in recent years. Objective: This study aims to develop a rapid enzyme immunoassay that can be applied to the quantitative analysis of Kwa in P. candollei and its derived products. Material and Method: A rapid and sensitive immunoassay was developed with a combination of Kwa-specific monoclonal antibody (MAb 11F) and Kwa–magnetic particles (MPs) conjugates, which increased the surface area of the solid phase, resulting in a decrease in the immunoreaction time. Result: This novel MPs-based enzyme immunoassay (MPs-EIA) was used to determine Kwa concentration in the range from 2.44 to 78.1 ng/mL with a limit of detection of 1.90 ng/mL. Validation analyses revealed that the proposed MPs-EIA protocol was sufficiently precise and accurate for effective quantitative analysis of Kwa in P. candollei and its derived products.
AB - Introduction: Kwakhurin (Kwa) is one of the unique isoflavonoids produced in Pueraria candollei var. mirifica (P. candollei), which has long been used as folk medicine for rejuvenation in Thailand. Recently, the use of P. candollei-derived products has widely spread among Japanese women for cosmetic purposes. Correspondingly, there has been an increase in the number of reports regarding possible health hazards caused by estrogenic activity inherent to the plant; thus, the need for a detailed evaluation of the phytoestrogen content of P. candollei-derived products has gained a sense of urgency in recent years. Objective: This study aims to develop a rapid enzyme immunoassay that can be applied to the quantitative analysis of Kwa in P. candollei and its derived products. Material and Method: A rapid and sensitive immunoassay was developed with a combination of Kwa-specific monoclonal antibody (MAb 11F) and Kwa–magnetic particles (MPs) conjugates, which increased the surface area of the solid phase, resulting in a decrease in the immunoreaction time. Result: This novel MPs-based enzyme immunoassay (MPs-EIA) was used to determine Kwa concentration in the range from 2.44 to 78.1 ng/mL with a limit of detection of 1.90 ng/mL. Validation analyses revealed that the proposed MPs-EIA protocol was sufficiently precise and accurate for effective quantitative analysis of Kwa in P. candollei and its derived products.
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U2 - 10.1002/pca.2964
DO - 10.1002/pca.2964
M3 - Article
C2 - 32542923
AN - SCOPUS:85086473337
SN - 0958-0344
VL - 31
SP - 930
EP - 936
JO - Phytochemical Analysis
JF - Phytochemical Analysis
IS - 6
ER -