Kinetic study of substrate dependency for higher butanol production in acetone-butanol-ethanol fermentation

Hideaki Shinto, Yukihiro Tashiro, Genta Kobayashi, Tatsuya Sekiguchi, Taizo Hanai, Yuki Kuriya, Masahiro Okamoto, Kenji Sonomoto

Research output: Contribution to journalArticlepeer-review

76 Citations (Scopus)


A kinetic simulation model of acetone-butanol-ethanol (ABE) fermentation of xylose (ModelXYL) was proposed by substituting Embden-Meyerhof-Parnas (EMP) pathway equations in the glucose model (ModelGLC) by pentose phosphate (PP) pathway equations of xylose utilization. We estimated the equation parameters of the PP pathway and set other equation parameters to the same as those in ModelGLC, by which ModelXYL exhibited an r2 value of 0.901 between the experimental time course of metabolites with initial xylose concentrations ranging from 40.7 to 292 mM and the calculated values. The results with the developed model suggested that Clostridium saccharoperbutylacetonicum N1-4 has a robust metabolic network in acid- and solvent-producing pathways. Furthermore, sensitivity analysis revealed that slow substrate utilization would be effective for higher butanol production; this coincided with the experimental results. Therefore, we consider the proposed model to be one of the best kinetic simulation candidates describing the dynamic metabolite behavior in ABE production.

Original languageEnglish
Pages (from-to)1452-1461
Number of pages10
JournalProcess Biochemistry
Issue number12
Publication statusPublished - Dec 2008

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology


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