Kid depletion in mouse oocytes associated with multinucleated blastomere formation and inferior embryo development

Akiyoshi Egashira; Nobuhiko Yamauchi; Md Rashedul Islam; Kazuki Yamagami; Asami Tanaka; Hikaru Suyama; El Sharawy Mohamed El-Sayed; Shoji Tabata; Takashi Kuramoto

doi:10.1111/asj.12530

Kid depletion in mouse oocytes associated with multinucleated blastomere formation and inferior embryo development

Akiyoshi Egashira, Nobuhiko Yamauchi, Md Rashedul Islam, Kazuki Yamagami, Asami Tanaka, Hikaru Suyama, El Sharawy Mohamed El-Sayed, Shoji Tabata, Takashi Kuramoto

Animal and Marine Bioresource Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

This study investigated the knockdown (KD) of Kid on maturation developmental competence and multinucleation of mouse germinal vesicle (GV) oocytes after parthenogenetic activation. Data revealed that Kid messenger RNA (mRNA) was expressed in GV and MII stage oocyte and 1- and 2-cell embryos. Additionally, Kid mRNA expression in the Kid KD group decreased by nearly 46% compared to the control small interfering RNA (siRNA) groups. The rate of multinucleated embryos in the Kid KD group (52.4%) was significantly higher (P < 0.05) than the control siRNA group (4.7%). Finally, the developmental rates were significantly lower in the Kid siRNA group at > 4-cell stage (28.6% vs. 53.5%) and the blastocyst stage (2.4% vs. 23.3%) compared to the control siRNA groups. Suppression of Kid using siRNA caused multinucleation in early embryos with high frequency and it may increase 2- to 4-cell arrested embryos and reduce the developmental competence to blastocyst.

Original language	English
Pages (from-to)	1048-1054
Number of pages	7
Journal	Animal science journal = Nihon chikusan Gakkaiho
Volume	87
Issue number	8
DOIs	https://doi.org/10.1111/asj.12530
Publication status	Published - Aug 1 2016

All Science Journal Classification (ASJC) codes

Medicine(all)

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10.1111/asj.12530

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title = "Kid depletion in mouse oocytes associated with multinucleated blastomere formation and inferior embryo development",

abstract = "This study investigated the knockdown (KD) of Kid on maturation developmental competence and multinucleation of mouse germinal vesicle (GV) oocytes after parthenogenetic activation. Data revealed that Kid messenger RNA (mRNA) was expressed in GV and MII stage oocyte and 1- and 2-cell embryos. Additionally, Kid mRNA expression in the Kid KD group decreased by nearly 46% compared to the control small interfering RNA (siRNA) groups. The rate of multinucleated embryos in the Kid KD group (52.4%) was significantly higher (P < 0.05) than the control siRNA group (4.7%). Finally, the developmental rates were significantly lower in the Kid siRNA group at > 4-cell stage (28.6% vs. 53.5%) and the blastocyst stage (2.4% vs. 23.3%) compared to the control siRNA groups. Suppression of Kid using siRNA caused multinucleation in early embryos with high frequency and it may increase 2- to 4-cell arrested embryos and reduce the developmental competence to blastocyst.",

author = "Akiyoshi Egashira and Nobuhiko Yamauchi and Islam, {Md Rashedul} and Kazuki Yamagami and Asami Tanaka and Hikaru Suyama and El-Sayed, {El Sharawy Mohamed} and Shoji Tabata and Takashi Kuramoto",

note = "Publisher Copyright: {\textcopyright} 2016 Japanese Society of Animal Science.",

year = "2016",

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doi = "10.1111/asj.12530",

language = "English",

volume = "87",

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AU - Egashira, Akiyoshi

AU - Yamauchi, Nobuhiko

AU - Islam, Md Rashedul

AU - Yamagami, Kazuki

AU - Tanaka, Asami

AU - Suyama, Hikaru

AU - El-Sayed, El Sharawy Mohamed

AU - Tabata, Shoji

AU - Kuramoto, Takashi

PY - 2016/8/1

Y1 - 2016/8/1

N2 - This study investigated the knockdown (KD) of Kid on maturation developmental competence and multinucleation of mouse germinal vesicle (GV) oocytes after parthenogenetic activation. Data revealed that Kid messenger RNA (mRNA) was expressed in GV and MII stage oocyte and 1- and 2-cell embryos. Additionally, Kid mRNA expression in the Kid KD group decreased by nearly 46% compared to the control small interfering RNA (siRNA) groups. The rate of multinucleated embryos in the Kid KD group (52.4%) was significantly higher (P < 0.05) than the control siRNA group (4.7%). Finally, the developmental rates were significantly lower in the Kid siRNA group at > 4-cell stage (28.6% vs. 53.5%) and the blastocyst stage (2.4% vs. 23.3%) compared to the control siRNA groups. Suppression of Kid using siRNA caused multinucleation in early embryos with high frequency and it may increase 2- to 4-cell arrested embryos and reduce the developmental competence to blastocyst.

AB - This study investigated the knockdown (KD) of Kid on maturation developmental competence and multinucleation of mouse germinal vesicle (GV) oocytes after parthenogenetic activation. Data revealed that Kid messenger RNA (mRNA) was expressed in GV and MII stage oocyte and 1- and 2-cell embryos. Additionally, Kid mRNA expression in the Kid KD group decreased by nearly 46% compared to the control small interfering RNA (siRNA) groups. The rate of multinucleated embryos in the Kid KD group (52.4%) was significantly higher (P < 0.05) than the control siRNA group (4.7%). Finally, the developmental rates were significantly lower in the Kid siRNA group at > 4-cell stage (28.6% vs. 53.5%) and the blastocyst stage (2.4% vs. 23.3%) compared to the control siRNA groups. Suppression of Kid using siRNA caused multinucleation in early embryos with high frequency and it may increase 2- to 4-cell arrested embryos and reduce the developmental competence to blastocyst.

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Kid depletion in mouse oocytes associated with multinucleated blastomere formation and inferior embryo development

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