Keratin mRNA for detecting micrometastasis in cervical lymph nodes of oral cancer

Hiroyuki Hamakawa; Masakuni Fukuzumi; Yang Bao; Tomoki Sumida; Hiroaki Kayahara; Akiko Onishi; Kenichi Sogawa

doi:10.1016/S0304-3835(00)00574-7

Keratin mRNA for detecting micrometastasis in cervical lymph nodes of oral cancer

Hiroyuki Hamakawa, Masakuni Fukuzumi, Yang Bao, Tomoki Sumida, Hiroaki Kayahara, Akiko Onishi, Kenichi Sogawa

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

We studied three keratin (K) gene candidates, K13, K19, and K20 mRNAs, for detecting micrometastases in cervical lymph nodes (LNs) by reverse transcriptase-polymerase chain reaction (RT-PCR). Of 166 histologically metastasis-negative nodes, 24 micrometastatic LNs (14.4%) were detected based on K13 gene expression. Keratin 19 mRNA is an inadequate marker for the genetic diagnosis due to not only illegitimate gene expression from lymphatic tissue but also gene expression from the ectopic salivary gland. Keratin 20 mRNA showed low sensitivity. It is suggested that K13 mRNA may be a promising tumor marker among these keratin genes for detecting the micrometastases in cervical LNs of oral cancer. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original language	English
Pages (from-to)	115-123
Number of pages	9
Journal	Cancer Letters
Volume	160
Issue number	1
DOIs	https://doi.org/10.1016/S0304-3835(00)00574-7
Publication status	Published - Nov 10 2000
Externally published	Yes

All Science Journal Classification (ASJC) codes

Oncology
Cancer Research

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0304-3835(00)00574-7

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title = "Keratin mRNA for detecting micrometastasis in cervical lymph nodes of oral cancer",

abstract = "We studied three keratin (K) gene candidates, K13, K19, and K20 mRNAs, for detecting micrometastases in cervical lymph nodes (LNs) by reverse transcriptase-polymerase chain reaction (RT-PCR). Of 166 histologically metastasis-negative nodes, 24 micrometastatic LNs (14.4%) were detected based on K13 gene expression. Keratin 19 mRNA is an inadequate marker for the genetic diagnosis due to not only illegitimate gene expression from lymphatic tissue but also gene expression from the ectopic salivary gland. Keratin 20 mRNA showed low sensitivity. It is suggested that K13 mRNA may be a promising tumor marker among these keratin genes for detecting the micrometastases in cervical LNs of oral cancer. Copyright (C) 2000 Elsevier Science Ireland Ltd.",

author = "Hiroyuki Hamakawa and Masakuni Fukuzumi and Yang Bao and Tomoki Sumida and Hiroaki Kayahara and Akiko Onishi and Kenichi Sogawa",

note = "Funding Information: This research was partially supported by the Ministry of Education, Science, Sports and Culture, Grant-in-Aid for Scientific Research (B), 10470434, 1998.",

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doi = "10.1016/S0304-3835(00)00574-7",

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T1 - Keratin mRNA for detecting micrometastasis in cervical lymph nodes of oral cancer

AU - Hamakawa, Hiroyuki

AU - Fukuzumi, Masakuni

AU - Bao, Yang

AU - Sumida, Tomoki

AU - Kayahara, Hiroaki

AU - Onishi, Akiko

AU - Sogawa, Kenichi

N1 - Funding Information: This research was partially supported by the Ministry of Education, Science, Sports and Culture, Grant-in-Aid for Scientific Research (B), 10470434, 1998.

PY - 2000/11/10

Y1 - 2000/11/10

N2 - We studied three keratin (K) gene candidates, K13, K19, and K20 mRNAs, for detecting micrometastases in cervical lymph nodes (LNs) by reverse transcriptase-polymerase chain reaction (RT-PCR). Of 166 histologically metastasis-negative nodes, 24 micrometastatic LNs (14.4%) were detected based on K13 gene expression. Keratin 19 mRNA is an inadequate marker for the genetic diagnosis due to not only illegitimate gene expression from lymphatic tissue but also gene expression from the ectopic salivary gland. Keratin 20 mRNA showed low sensitivity. It is suggested that K13 mRNA may be a promising tumor marker among these keratin genes for detecting the micrometastases in cervical LNs of oral cancer. Copyright (C) 2000 Elsevier Science Ireland Ltd.

AB - We studied three keratin (K) gene candidates, K13, K19, and K20 mRNAs, for detecting micrometastases in cervical lymph nodes (LNs) by reverse transcriptase-polymerase chain reaction (RT-PCR). Of 166 histologically metastasis-negative nodes, 24 micrometastatic LNs (14.4%) were detected based on K13 gene expression. Keratin 19 mRNA is an inadequate marker for the genetic diagnosis due to not only illegitimate gene expression from lymphatic tissue but also gene expression from the ectopic salivary gland. Keratin 20 mRNA showed low sensitivity. It is suggested that K13 mRNA may be a promising tumor marker among these keratin genes for detecting the micrometastases in cervical LNs of oral cancer. Copyright (C) 2000 Elsevier Science Ireland Ltd.

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Keratin mRNA for detecting micrometastasis in cervical lymph nodes of oral cancer

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

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