Keratin mRNA for detecting micrometastasis in cervical lymph nodes of oral cancer

Hiroyuki Hamakawa, Masakuni Fukuzumi, Yang Bao, Tomoki Sumida, Hiroaki Kayahara, Akiko Onishi, Kenichi Sogawa

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)


We studied three keratin (K) gene candidates, K13, K19, and K20 mRNAs, for detecting micrometastases in cervical lymph nodes (LNs) by reverse transcriptase-polymerase chain reaction (RT-PCR). Of 166 histologically metastasis-negative nodes, 24 micrometastatic LNs (14.4%) were detected based on K13 gene expression. Keratin 19 mRNA is an inadequate marker for the genetic diagnosis due to not only illegitimate gene expression from lymphatic tissue but also gene expression from the ectopic salivary gland. Keratin 20 mRNA showed low sensitivity. It is suggested that K13 mRNA may be a promising tumor marker among these keratin genes for detecting the micrometastases in cervical LNs of oral cancer. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)115-123
Number of pages9
JournalCancer Letters
Issue number1
Publication statusPublished - Nov 10 2000
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research


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