TY - JOUR
T1 - Kat1-antigen - A reliable immunological marker for identifying osteoclast precursors of rats
T2 - Detection of subpopulations among precursors and initiation of osteoclastogenesis
AU - Kukita, Toshio
AU - Kukita, Akiko
AU - Xu, Licin
AU - Watanabe, Toshiyuki
AU - Iijima, Tadahiko
N1 - Funding Information:
Acknowledgements We thank Dr. Toshihiko Koga, Faculty of Dental Science, Kyushu University, for his encouragement. We also thank Dr. Hiroshi Harada, Faculty of Medicine, Kyushu University, for discussion. This work was partly supported by a grant for scientific research from the Japanese Ministry of Education, Science, Sports, and Culture (project number 06671816).
PY - 2001
Y1 - 2001
N2 - Previously we found a unique cell surface antigen [Kat1-antigen (Kat1-Ag)] expressed on rat osteoclasts. In the present study, we focused on the expression of this antigen in preosteoclasts, mononuclear precursors of osteoclasts. Immunohistochemical and immunoelectron microscopic observations of the Kat1-Ag expressed in vivo showed the antigen to be present on mononuclear cells having the morphological features of preosteoclasts. The relationship between Kat1-Ag expression and calcitonin receptor (CTR) expression was examined in detail by a double-detection technique for CTR and Kat1-Ag by use of autoradiography and immunocytochemistry, respectively. In a culture system for forming mononuclear preosteoclast-like cells, almost 100% of the mononuclear cells expressing Kat1-Ag also expressed CTR, demonstrating that Kat1-Ag is a reliable immunological marker for identifying preosteoclasts. Interestingly, a significant number of the CTR-positive mononuclear cells did not express the Kat1-Ag. Detection of these cells expressing CTR but not Kat1-Ag strongly suggests the presence of subpopulations in preosteoclasts. We also obtained evidence suggesting that expression of the Kat1-Ag is initiated during the postmitotic stage of the osteoclast progenitors.
AB - Previously we found a unique cell surface antigen [Kat1-antigen (Kat1-Ag)] expressed on rat osteoclasts. In the present study, we focused on the expression of this antigen in preosteoclasts, mononuclear precursors of osteoclasts. Immunohistochemical and immunoelectron microscopic observations of the Kat1-Ag expressed in vivo showed the antigen to be present on mononuclear cells having the morphological features of preosteoclasts. The relationship between Kat1-Ag expression and calcitonin receptor (CTR) expression was examined in detail by a double-detection technique for CTR and Kat1-Ag by use of autoradiography and immunocytochemistry, respectively. In a culture system for forming mononuclear preosteoclast-like cells, almost 100% of the mononuclear cells expressing Kat1-Ag also expressed CTR, demonstrating that Kat1-Ag is a reliable immunological marker for identifying preosteoclasts. Interestingly, a significant number of the CTR-positive mononuclear cells did not express the Kat1-Ag. Detection of these cells expressing CTR but not Kat1-Ag strongly suggests the presence of subpopulations in preosteoclasts. We also obtained evidence suggesting that expression of the Kat1-Ag is initiated during the postmitotic stage of the osteoclast progenitors.
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U2 - 10.1007/s004180100248
DO - 10.1007/s004180100248
M3 - Article
C2 - 11326749
AN - SCOPUS:0035084231
SN - 0948-6143
VL - 115
SP - 215
EP - 222
JO - Histochemistry and Cell Biology
JF - Histochemistry and Cell Biology
IS - 3
ER -