Jmjd3 controls mesodermal and cardiovascular differentiation of embryonic stem cells

Kisho Ohtani; Cong Zhao; Gergana Dobreva; Yosif Manavski; Britta Kluge; Thomas Braun; Michael A. Rieger; Andreas M. Zeiher; Stefanie Dimmeler

doi:10.1161/CIRCRESAHA.113.302035

Jmjd3 controls mesodermal and cardiovascular differentiation of embryonic stem cells

Kisho Ohtani, Cong Zhao, Gergana Dobreva, Yosif Manavski, Britta Kluge, Thomas Braun, Michael A. Rieger, Andreas M. Zeiher, Stefanie Dimmeler

Research output: Contribution to journal › Article › peer-review

68 Citations (Scopus)

Abstract

RATIONALE:: The developmental role of the H3K27 demethylases Jmjd3, especially its epigenetic regulation at target genes in response to upstream developmental signaling, is unclear. OBJECTIVE:: To determine the role of Jmjd3 during mesoderm and cardiovascular lineage commitment. METHODS AND RESULTS:: Ablation of Jmjd3 in mouse embryonic stem cells does not affect the maintenance of pluripotency and self-renewal but compromised mesoderm and subsequent endothelial and cardiac differentiation. Jmjd3 reduces H3K27me3 marks at the Brachyury promoter and facilitates the recruitment of β-catenin, which is critical for Wnt signal-induced mesoderm differentiation. CONCLUSIONS:: These data demonstrate that Jmjd3 is required for mesoderm differentiation and cardiovascular lineage commitment.

Original language	English
Pages (from-to)	856-862
Number of pages	7
Journal	Circulation research
Volume	113
Issue number	7
DOIs	https://doi.org/10.1161/CIRCRESAHA.113.302035
Publication status	Published - Sept 13 2013
Externally published	Yes

All Science Journal Classification (ASJC) codes

Physiology
Cardiology and Cardiovascular Medicine

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10.1161/CIRCRESAHA.113.302035

Cite this

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title = "Jmjd3 controls mesodermal and cardiovascular differentiation of embryonic stem cells",

abstract = "RATIONALE:: The developmental role of the H3K27 demethylases Jmjd3, especially its epigenetic regulation at target genes in response to upstream developmental signaling, is unclear. OBJECTIVE:: To determine the role of Jmjd3 during mesoderm and cardiovascular lineage commitment. METHODS AND RESULTS:: Ablation of Jmjd3 in mouse embryonic stem cells does not affect the maintenance of pluripotency and self-renewal but compromised mesoderm and subsequent endothelial and cardiac differentiation. Jmjd3 reduces H3K27me3 marks at the Brachyury promoter and facilitates the recruitment of β-catenin, which is critical for Wnt signal-induced mesoderm differentiation. CONCLUSIONS:: These data demonstrate that Jmjd3 is required for mesoderm differentiation and cardiovascular lineage commitment.",

author = "Kisho Ohtani and Cong Zhao and Gergana Dobreva and Yosif Manavski and Britta Kluge and Thomas Braun and Rieger, {Michael A.} and Zeiher, {Andreas M.} and Stefanie Dimmeler",

year = "2013",

month = sep,

day = "13",

doi = "10.1161/CIRCRESAHA.113.302035",

language = "English",

volume = "113",

pages = "856--862",

journal = "Circulation research",

issn = "0009-7330",

publisher = "Lippincott Williams and Wilkins",

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TY - JOUR

T1 - Jmjd3 controls mesodermal and cardiovascular differentiation of embryonic stem cells

AU - Ohtani, Kisho

AU - Zhao, Cong

AU - Dobreva, Gergana

AU - Manavski, Yosif

AU - Kluge, Britta

AU - Braun, Thomas

AU - Rieger, Michael A.

AU - Zeiher, Andreas M.

AU - Dimmeler, Stefanie

PY - 2013/9/13

Y1 - 2013/9/13

N2 - RATIONALE:: The developmental role of the H3K27 demethylases Jmjd3, especially its epigenetic regulation at target genes in response to upstream developmental signaling, is unclear. OBJECTIVE:: To determine the role of Jmjd3 during mesoderm and cardiovascular lineage commitment. METHODS AND RESULTS:: Ablation of Jmjd3 in mouse embryonic stem cells does not affect the maintenance of pluripotency and self-renewal but compromised mesoderm and subsequent endothelial and cardiac differentiation. Jmjd3 reduces H3K27me3 marks at the Brachyury promoter and facilitates the recruitment of β-catenin, which is critical for Wnt signal-induced mesoderm differentiation. CONCLUSIONS:: These data demonstrate that Jmjd3 is required for mesoderm differentiation and cardiovascular lineage commitment.

AB - RATIONALE:: The developmental role of the H3K27 demethylases Jmjd3, especially its epigenetic regulation at target genes in response to upstream developmental signaling, is unclear. OBJECTIVE:: To determine the role of Jmjd3 during mesoderm and cardiovascular lineage commitment. METHODS AND RESULTS:: Ablation of Jmjd3 in mouse embryonic stem cells does not affect the maintenance of pluripotency and self-renewal but compromised mesoderm and subsequent endothelial and cardiac differentiation. Jmjd3 reduces H3K27me3 marks at the Brachyury promoter and facilitates the recruitment of β-catenin, which is critical for Wnt signal-induced mesoderm differentiation. CONCLUSIONS:: These data demonstrate that Jmjd3 is required for mesoderm differentiation and cardiovascular lineage commitment.

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JO - Circulation research

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Jmjd3 controls mesodermal and cardiovascular differentiation of embryonic stem cells

Abstract

All Science Journal Classification (ASJC) codes

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