TY - JOUR
T1 - Isolation of arabidopsis ahg11, a weak ABA hypersensitive mutant defective in nad4 RNA editing
AU - Murayama, Maki
AU - Hayashi, Shimpei
AU - Nishimura, Noriyuki
AU - Ishide, Mayumi
AU - Kobayashi, Keiko
AU - Yagi, Yusuke
AU - Asami, Tadao
AU - Nakamura, Takahiro
AU - Shinozaki, Kazuo
AU - Hirayama, Takashi
N1 - Funding Information:
This work was supported in part by an Incentive Research Grant from RIKEN ASI, Grants-in-Aid for Scientific Research (C) (20570050), Grants-in-Aid for Scientific Research on Innovative Areas (RNA regulation) (21112521, 23112715) from MEXT, and the Ohara Foundation for Agricultural Science to TH. Experiments using confocal laser scanning microscope in this study was assisted by the Japan Advance Plant Science Network.
PY - 2012/9
Y1 - 2012/9
N2 - The phytohormone abscisic acid (ABA) plays pivotal roles in the regulation of developmental and environmental responses in plants. Identification of cytoplasmic ABA receptors enabled the elucidation of the main ABA signalling pathway, connecting ABA perception to either nuclear events or the action of several transporters. However, the physiological functions of ABA in cellular processes largely remain unknown. To obtain greater insight into the ABA response, genetic screening was performed to isolate ABA-related mutants of Arabidopsis and several novel ABA-hypersensitive mutants were isolated. One of those mutantsahg11was characterized further. Map-based cloning showed that AHG11 encodes a PPR type protein, which has potential roles in RNA editing. An AHG11-GFP fusion protein indicated that AHG11 mainly localized to the mitochondria. Consistent with this observation, the nad4 transcript, which normally undergoes RNA editing, lacks a single RNA editing event conferring a conversion of an amino acid residue in ahg11 mutants. The geminating ahg11 seeds have higher levels of reactive-oxygen-species-responsive genes. Presumably, partial impairment of mitochondrial function caused by an amino acid conversion in one of the complex I components induces redox imbalance which, in turn, confers an abnormal response to the plant hormone.
AB - The phytohormone abscisic acid (ABA) plays pivotal roles in the regulation of developmental and environmental responses in plants. Identification of cytoplasmic ABA receptors enabled the elucidation of the main ABA signalling pathway, connecting ABA perception to either nuclear events or the action of several transporters. However, the physiological functions of ABA in cellular processes largely remain unknown. To obtain greater insight into the ABA response, genetic screening was performed to isolate ABA-related mutants of Arabidopsis and several novel ABA-hypersensitive mutants were isolated. One of those mutantsahg11was characterized further. Map-based cloning showed that AHG11 encodes a PPR type protein, which has potential roles in RNA editing. An AHG11-GFP fusion protein indicated that AHG11 mainly localized to the mitochondria. Consistent with this observation, the nad4 transcript, which normally undergoes RNA editing, lacks a single RNA editing event conferring a conversion of an amino acid residue in ahg11 mutants. The geminating ahg11 seeds have higher levels of reactive-oxygen-species-responsive genes. Presumably, partial impairment of mitochondrial function caused by an amino acid conversion in one of the complex I components induces redox imbalance which, in turn, confers an abnormal response to the plant hormone.
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U2 - 10.1093/jxb/ers188
DO - 10.1093/jxb/ers188
M3 - Article
C2 - 22821940
AN - SCOPUS:84865803387
SN - 0022-0957
VL - 63
SP - 5301
EP - 5310
JO - Journal of Experimental Botany
JF - Journal of Experimental Botany
IS - 14
ER -