TY - JOUR
T1 - Isolation of a T cell specific cDNA clone possibly involved in the T cell activation pathway
AU - Takihara, Yoshlhiro
AU - Caccia, Nicolette
AU - Yanagi, Yusuke
AU - Mak, Tak W.
N1 - Funding Information:
We wish to acknowledge Dr A Weiss for providing us with the Northern filter shown in Figure 4. We thank Drs Alklchi Iwamoto, Yasuhiro Koga, and Pamela S. Ohashi for helpful comments during the course of the experiments. This work Is supported by the MRC, NSERC, NCI of Canada and a special research grant from the University of Toronto. Y.T. Is a recipient of an award from the MRC of Canada and N.C. is a recipient of an award from NCI of Canada.
PY - 1989
Y1 - 1989
N2 - A T cell specific cDNA clone, YT15, has been isolated using the technique of subtractive hybrldlzation. This gene is expressed predominantly in thymocytes and encodes a newly identified Intracytoplasmic protein specific. for T cells, since neither the nucleotide sequence of the cDNA clone nor the deduced amino acid sequence has significant homology to sequences in the GenBank or NBRF databases. The level of transcription of this gene in human leukemlc call line, Jurkat, can be reduced by stimulation of the T cell receptor CD3 (TcR-T3) complex. In addition, the levels of these messages are also greatly enhanced in some TcR/T3 receptor mutants. These findings suggest that this gene product may be involved in a T call activation pathway within the cell.
AB - A T cell specific cDNA clone, YT15, has been isolated using the technique of subtractive hybrldlzation. This gene is expressed predominantly in thymocytes and encodes a newly identified Intracytoplasmic protein specific. for T cells, since neither the nucleotide sequence of the cDNA clone nor the deduced amino acid sequence has significant homology to sequences in the GenBank or NBRF databases. The level of transcription of this gene in human leukemlc call line, Jurkat, can be reduced by stimulation of the T cell receptor CD3 (TcR-T3) complex. In addition, the levels of these messages are also greatly enhanced in some TcR/T3 receptor mutants. These findings suggest that this gene product may be involved in a T call activation pathway within the cell.
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U2 - 10.1093/intimm/1.1.59
DO - 10.1093/intimm/1.1.59
M3 - Article
C2 - 2487676
AN - SCOPUS:0024461516
SN - 0953-8178
VL - 1
SP - 59
EP - 65
JO - International immunology
JF - International immunology
IS - 1
ER -