TY - JOUR
T1 - Isolation of a new peroxisome-deficient CHO cell mutant defective in peroxisome targeting signal-1 receptor
AU - Tsukamoto, Toshiro
AU - Bogaki, Akemi
AU - Okumoto, Kanji
AU - Tateishi, Keita
AU - Fujiki, Yukio
AU - Shimozawa, Nobuyuki
AU - Suzuki, Yasuyuki
AU - Kondo, Naomi
AU - Osumi, Takashi
N1 - Funding Information:
This work was supported in part by grants from the Ministry of Education, Science, and Culture of Japan, The Sumitomo Foundation, and Uehara Memorial Foundation.
PY - 1997/1/13
Y1 - 1997/1/13
N2 - For the study of mechanism of peroxisome biogenesis, we attempted to isolate CHO cell mutants deficient in peroxisome biogenesis. We used as the parent strain a stable CHO transformant of rat PEX2 (formerly named peroxisome assembly factor-1) cDNA, to avoid unusually frequent isolation of Pex2 mutants. Among the three peroxisome-deficient mutants obtained, ZP102 was a new CHO mutant of complementation group 2, and was restored for peroxisome assembly by the transfection of human PEX5 (formerly called PXR1 or PTS1R) cDNA. This approach would facilitate the isolation of new complementation gorups of peroxisome-deficient CHO mutants and the identification of essential genes for peroxisome biogenesis.
AB - For the study of mechanism of peroxisome biogenesis, we attempted to isolate CHO cell mutants deficient in peroxisome biogenesis. We used as the parent strain a stable CHO transformant of rat PEX2 (formerly named peroxisome assembly factor-1) cDNA, to avoid unusually frequent isolation of Pex2 mutants. Among the three peroxisome-deficient mutants obtained, ZP102 was a new CHO mutant of complementation group 2, and was restored for peroxisome assembly by the transfection of human PEX5 (formerly called PXR1 or PTS1R) cDNA. This approach would facilitate the isolation of new complementation gorups of peroxisome-deficient CHO mutants and the identification of essential genes for peroxisome biogenesis.
UR - http://www.scopus.com/inward/record.url?scp=0031566178&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031566178&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1996.5971
DO - 10.1006/bbrc.1996.5971
M3 - Article
C2 - 9016792
AN - SCOPUS:0031566178
SN - 0006-291X
VL - 230
SP - 402
EP - 406
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -