Isolation of a carp complement protein homologous to mammalian factor D

Yano Tomoki, Nakao Miki

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)


Factor D of carp (Cyprinus carpio) complement was purified to apparent homogeneity by a 4-step Chromatographie procedure and examined for physicochemical and functional properties. Carp factor D proved to be an α-globulin with a molecular mass of 29 kDa and the serum concentration was estimated to be 6 μg/ml. The NH2-terminal amino acid sequence (30 residues including five unidentified positions) of carp factor D showed high homologies (57-60%) to those of mammalian factor D. Neither functional compatibility nor common antigenicity was observed between carp and human factor D. This report is apparently the first description on the factor D molecule in a non-mammalian species.

Original languageEnglish
Pages (from-to)337-342
Number of pages6
JournalMolecular Immunology
Issue number5
Publication statusPublished - Apr 1994

All Science Journal Classification (ASJC) codes

  • Immunology
  • Molecular Biology


Dive into the research topics of 'Isolation of a carp complement protein homologous to mammalian factor D'. Together they form a unique fingerprint.

Cite this