Isolation and characterization of microsatellite markers from Robinia pseudoacacia L.

Kentaro Mishima; Tomonori Hirao; Shougo Urano; Atsushi Watanabe; Katsuhiko Takata

doi:10.1111/j.1755-0998.2008.02306.x

Isolation and characterization of microsatellite markers from Robinia pseudoacacia L.

Kentaro Mishima, Tomonori Hirao, Shougo Urano, Atsushi Watanabe, Katsuhiko Takata

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Microsatellite markers were isolated from Robinia pseudoacacia L. using an enrichment method. Eleven of the 23 primer pairs designed successfully amplified unambiguous and polymorphic single loci among 39 individual R. pseudoacacia L. from northeastern Japan. The observed and expected heterozygosities of the 11 microsatellite markers ranged from 0.333 to 0.821 and from 0.489 to 0.867, respectively. The polymorphisms observed at the 11 microsatellite loci are useful genetic data for forest ecological studies involving R. pseudoacacia L.

Original language	English
Pages (from-to)	850-852
Number of pages	3
Journal	Molecular Ecology Resources
Volume	9
Issue number	3
DOIs	https://doi.org/10.1111/j.1755-0998.2008.02306.x
Publication status	Published - May 2009
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Ecology, Evolution, Behavior and Systematics
Genetics

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10.1111/j.1755-0998.2008.02306.x

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abstract = "Microsatellite markers were isolated from Robinia pseudoacacia L. using an enrichment method. Eleven of the 23 primer pairs designed successfully amplified unambiguous and polymorphic single loci among 39 individual R. pseudoacacia L. from northeastern Japan. The observed and expected heterozygosities of the 11 microsatellite markers ranged from 0.333 to 0.821 and from 0.489 to 0.867, respectively. The polymorphisms observed at the 11 microsatellite loci are useful genetic data for forest ecological studies involving R. pseudoacacia L.",

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AU - Hirao, Tomonori

AU - Urano, Shougo

AU - Watanabe, Atsushi

AU - Takata, Katsuhiko

PY - 2009/5

Y1 - 2009/5

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AB - Microsatellite markers were isolated from Robinia pseudoacacia L. using an enrichment method. Eleven of the 23 primer pairs designed successfully amplified unambiguous and polymorphic single loci among 39 individual R. pseudoacacia L. from northeastern Japan. The observed and expected heterozygosities of the 11 microsatellite markers ranged from 0.333 to 0.821 and from 0.489 to 0.867, respectively. The polymorphisms observed at the 11 microsatellite loci are useful genetic data for forest ecological studies involving R. pseudoacacia L.

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Isolation and characterization of microsatellite markers from Robinia pseudoacacia L.

Abstract

All Science Journal Classification (ASJC) codes

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