PCR was used to isolate an invertase homolog gene from the fission yeast Schizosaccharomyces pombe. The cloned inv1+ gene encodes a protein of 581 amino acids with 16 potential asparagine-linked glycosylation sites, and has 39% and 38% identity to the Schwanniomyces occidentalis and Saccharomyces cerevisiae SUC2 invertases. When the inv1+ gene was disrupted, S. pombe strains lacked detectable invertase activity. This result showed that the inv1+ gene encodes only one active invertase in S. pombe cells. The transcription of inv1+ is repressed in the presence of glucose. The transcription of inv1+ was not affected in cyr1Δ strain which lacks adenylate cyclase activity, unlike transcription of S. pombe fbp1+ gene. We have identified an S. pombe gene (scr1+) that encodes a homolog of the Aspergillus nidulans CREA which is required for glucose repression of the glyconeogenic pathway. Although the deletion of scr1+ did not influence the transcription of fbp1+ gene, glucose repression of the inv1+ gene was severely affected. These results showed that glucose repression of inv1+ gene is dependent on scr1+ gene, and S. pombe cAMP signalling pathway may not be essential for glucose repression of inv1+ gene.
|Number of pages||8|
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - Apr 7 1998|
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology