TY - JOUR
T1 - Isolation and characterization of 11 polymorphic microsatellite loci in the whitegirdled goby (Pterogobius zonoleucus) and cross-species amplification in the serpentine goby (P. elapoides)
AU - Nohara, K.
AU - Kokita, T.
AU - Tominaga, O.
AU - Seikai, T.
PY - 2009/3
Y1 - 2009/3
N2 - Eleven polymorphic microsatellite markers were developed from a (CA) n-enrichment library of the whitegirdled goby (Pterogobius zonoleucus). Polymorphism at these loci ranged from 2 to 12 alleles, and observed and expected heterozygosities from 0.05 to 0.90 and from 0.05 and 0.86, respectively. All loci conformed to Hardy-Weinberg equilibrium, with no significant linkage disequilibrium between all locus pairs. Cross-species amplification tests were successful in P. elapoides, and most loci were polymorphic. These microsatellite markers will be useful in further population genetic studies of both species.
AB - Eleven polymorphic microsatellite markers were developed from a (CA) n-enrichment library of the whitegirdled goby (Pterogobius zonoleucus). Polymorphism at these loci ranged from 2 to 12 alleles, and observed and expected heterozygosities from 0.05 to 0.90 and from 0.05 and 0.86, respectively. All loci conformed to Hardy-Weinberg equilibrium, with no significant linkage disequilibrium between all locus pairs. Cross-species amplification tests were successful in P. elapoides, and most loci were polymorphic. These microsatellite markers will be useful in further population genetic studies of both species.
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U2 - 10.1111/j.1471-8286.2009.00228_9_2.x
DO - 10.1111/j.1471-8286.2009.00228_9_2.x
M3 - Article
AN - SCOPUS:60349115201
SN - 1755-098X
VL - 9
SP - 610
EP - 612
JO - Molecular Ecology Resources
JF - Molecular Ecology Resources
IS - 2
ER -