TY - JOUR
T1 - Involvement of the T-box transcription factor Brachyury in early-stage embryonic mouse salivary gland
AU - Hayashi, Kouhei
AU - Ikari, Tatsuya
AU - Sugiyama, Goro
AU - Sugiura, Tsuyoshi
AU - Ohyama, Yukiko
AU - Kumamaru, Wataru
AU - Shirasuna, Kanemitsu
AU - Mori, Yoshihide
N1 - Funding Information:
This research was supported by a Grant-in-Aid (KAKEN No. 24792144 ) in Japan Society for the Promotion of Science (to T. Ikari).
Publisher Copyright:
© 2016 Elsevier Inc.
PY - 2016/9/2
Y1 - 2016/9/2
N2 - The mouse submandibular gland (SMG) is important organ for embryonic development, and branching morphogenesis is regulated by many molecules containing transcription factors. Real-time reverse transcriptase polymerase chain reaction revealed that the expression of Brachyury increased in the SMG and peaked between E12.5–E13.5, concomitant with the early stage of branching morphogenesis. The expression of Brachyury in SMG rudiments between E12.5–E13.5 was confirmed by western blotting. In addition, fibronectin and Btbd7 (regulated by fibronectin), which are both essential for cleft formation, were expressed strongly during the same period. The Sox2 and Wnt3a, which regulate cell growth, were also expressed strongly during E12.5–E13.5. On the other hand, cleft formation and branching morphogenesis was suppressed by knockdown of Brachyury gene, suggesting that Brachyury plays a central role in regulating cell growth and cleft formation in early-stage embryonic mouse salivary gland development.
AB - The mouse submandibular gland (SMG) is important organ for embryonic development, and branching morphogenesis is regulated by many molecules containing transcription factors. Real-time reverse transcriptase polymerase chain reaction revealed that the expression of Brachyury increased in the SMG and peaked between E12.5–E13.5, concomitant with the early stage of branching morphogenesis. The expression of Brachyury in SMG rudiments between E12.5–E13.5 was confirmed by western blotting. In addition, fibronectin and Btbd7 (regulated by fibronectin), which are both essential for cleft formation, were expressed strongly during the same period. The Sox2 and Wnt3a, which regulate cell growth, were also expressed strongly during E12.5–E13.5. On the other hand, cleft formation and branching morphogenesis was suppressed by knockdown of Brachyury gene, suggesting that Brachyury plays a central role in regulating cell growth and cleft formation in early-stage embryonic mouse salivary gland development.
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U2 - 10.1016/j.bbrc.2016.06.140
DO - 10.1016/j.bbrc.2016.06.140
M3 - Article
C2 - 27369076
AN - SCOPUS:84979649937
SN - 0006-291X
VL - 477
SP - 814
EP - 819
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -