TY - JOUR
T1 - Involvement of M1/M2 Macrophage Polarization in Reparative Dentin Formation
AU - Kadowaki, Masataka
AU - Yoshida, Shinichiro
AU - Itoyama, Tomohiro
AU - Tomokiyo, Atsushi
AU - Hamano, Sayuri
AU - Hasegawa, Daigaku
AU - Sugii, Hideki
AU - Kaneko, Hiroshi
AU - Sugiura, Risa
AU - Maeda, Hidefumi
N1 - Funding Information:
This study was financially supported by the Grants-in Aid for Scientific research (JP17H01598, JP20K18538, and JP22K17061) from the Japan Society for the Promotion of Science and JST Support for Pioneering Research Initiated by the Next Generation (JPMJSP2136). We also thank Helen Jeays, BDSc AE, from Edanz ( https://jp.edanz.com/ac ) (4 August 2022) for editing a draft of this manuscript.
Publisher Copyright:
© 2022 by the authors.
PY - 2022/11
Y1 - 2022/11
N2 - In cases in which dental pulp tissue is accidentally exposed, direct pulp capping is often performed to induce reparative dentin formation. Although macrophages are essential for the inflammatory response and tissue repair, the emergence pattern and the role of macrophages in dental pulp tissue have not been clarified. Here, we investigated the emergence of M1/M2 macrophages in dental pulp tissue after a direct pulp capping and the effects of M2 macrophages on odontoblastic differentiation of the dental pulp stem cell (DPSC) clones. The emergence of macrophages in dental pulp tissue was investigated using a rat direct pulp capping model. Alizarin Red S staining and quantitative RT-PCR was performed to examine the effect of M2 macrophages on the mineralization and odontoblastic differentiation of DPSC clones. Immunohistochemical staining revealed that M1 macrophages were detected in dental pulp tissue after treatment and increased in number at three days after treatment. However, M2 macrophages gradually increased in number in dental pulp tissue after treatment, with the highest level recorded at seven days post-operation. Additionally, conditioned medium from M2 macrophages induced odontoblast-like differentiation of DPSC clones. These results suggest that macrophages play a role in the inflammatory response and reparative dentin formation after dental pulp exposure.
AB - In cases in which dental pulp tissue is accidentally exposed, direct pulp capping is often performed to induce reparative dentin formation. Although macrophages are essential for the inflammatory response and tissue repair, the emergence pattern and the role of macrophages in dental pulp tissue have not been clarified. Here, we investigated the emergence of M1/M2 macrophages in dental pulp tissue after a direct pulp capping and the effects of M2 macrophages on odontoblastic differentiation of the dental pulp stem cell (DPSC) clones. The emergence of macrophages in dental pulp tissue was investigated using a rat direct pulp capping model. Alizarin Red S staining and quantitative RT-PCR was performed to examine the effect of M2 macrophages on the mineralization and odontoblastic differentiation of DPSC clones. Immunohistochemical staining revealed that M1 macrophages were detected in dental pulp tissue after treatment and increased in number at three days after treatment. However, M2 macrophages gradually increased in number in dental pulp tissue after treatment, with the highest level recorded at seven days post-operation. Additionally, conditioned medium from M2 macrophages induced odontoblast-like differentiation of DPSC clones. These results suggest that macrophages play a role in the inflammatory response and reparative dentin formation after dental pulp exposure.
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U2 - 10.3390/life12111812
DO - 10.3390/life12111812
M3 - Article
AN - SCOPUS:85141741418
SN - 0024-3019
VL - 12
JO - Life
JF - Life
IS - 11
M1 - 1812
ER -