Involvement of IMP dehydrogenase activity in induction of sporulation of Bacillus cereus

Takahisa Miyamoto, Makoto Imamura, Kiyoshi Matsuno, Sam In Kim, Ken-ichi Honjoh, Shoji Hatano

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2 Citations (Scopus)


IMP dehydrogenase activity of B. cereus increased parallel to cell growth in YE-EMM, where B. cereus did not sporulate. When B. cereus was cultured in a modified G medium, a sporulation medium, the activity reached the highest level at 6 hr and decreased thereafter. After induction of sporulation by nutritional shift down in 1/100 G medium, the enzyme activity decreased to about 5% compared with exponentially growing cells at 1 hr of resuspension. The sporulation rate of B. cereus was over 90% in the modified G medium and 1/100 G medium. Sporulation was strongly inhibited by mycophenolic acid at 1 mM, when the drug was added at 0 and 1 hr of resuspension in 1/100 G medium. Intracellular GTP concentration of B. cereus decreased to the lowest level about 1 hr of resuspension. Although GTP increased to about 50% of the exponentially growing cells at 2 hr of resuspension in control cells, the concentration did not increase in the presence of 1 mM mycophenolic acid.

Original languageEnglish
Pages (from-to)277-280
Number of pages4
JournalMicrobiological Research
Issue number3
Publication statusPublished - Sept 1997

All Science Journal Classification (ASJC) codes

  • Microbiology


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